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Cortisol, Free and Total, LC/MS
Test CodeFCORT
Quest Code
37077
CPT Codes
82530, 82533
Preferred Specimen
2 mL serum collected in a red-top tube (no gel) - See Collection Instructions
Minimum Volume
1.4 mL (0.7 mL in each tube)
Other Acceptable Specimens
Plasma collected in: EDTA (lavender-top) tube
Instructions
Collect 2 mL (1.4 mL minimum) serum or plasma and aliquot into two separate transport tubes, with 1 mL (0.7 mL minimum) in each transport tube.
Specify time of day specimen was collected.
Specify time of day specimen was collected.
Transport Container
Two separate transport tubes
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 1 year
Refrigerated: 7 days
Frozen: 1 year
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Received room temperature • Serum separator tube (SST)
Methodology
Equilibrium Dialysis • Chromatography/Mass Spectrometry
Reference Range
See Laboratory Report
Clinical Significance
Free cortisol is useful in the detection of patients with Cushing's syndrome for whom free cortisol concentrations are elevated.
Performing Laboratory
| Quest Diagnostics Nichols Institute-San Juan Capistrano, CA |
| 33608 Ortega Highway |
| San Juan Capistrano, CA 92675-2042 |
