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HLA-B High Resolution
Test Code17396
Preferred Specimen
14 mL whole blood collected in an EDTA (lavender-top) tube
Pediatric volume:
0-1 year: 3 mL
1-5 years: 5 mL
5-10 years: 7 mL
>10 years: 14 mL
Pediatric volume:
0-1 year: 3 mL
1-5 years: 5 mL
5-10 years: 7 mL
>10 years: 14 mL
Minimum Volume
5 mL
Other Acceptable Specimens
Whole blood collected in an: acid citrate dextrose ACD (yellow-top), or sodium or lithium heparin (green-top) tube • Minimum: 4 buccal swab
Instructions
Samples cannot be shipped on Saturday or the day before a holiday.
Other Acceptable sample types: For cord blood, bone marrow and peripheral blood leukocyte specimen submissions, please contact Versiti Wisconsin for collection, transport and stability instructions.
For Adult patients: If requesting more than one panel for HLA Transplant Testing, no more than 14 mL whole blood collected in EDTA (lavender-top) tubes is required.
For Pediatric patients: If requesting more than one panel for HLA Transplant Testing, follow drawing instructions according to age as specified. No more than what is specified by age is required.
Other Acceptable sample types: For cord blood, bone marrow and peripheral blood leukocyte specimen submissions, please contact Versiti Wisconsin for collection, transport and stability instructions.
For Adult patients: If requesting more than one panel for HLA Transplant Testing, no more than 14 mL whole blood collected in EDTA (lavender-top) tubes is required.
For Pediatric patients: If requesting more than one panel for HLA Transplant Testing, follow drawing instructions according to age as specified. No more than what is specified by age is required.
Transport Temperature
Room temperature
Specimen Stability
Whole blood (preferred)
Room temperature: 14 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Buccal swab
Room temperature: 30 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Room temperature: 14 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Buccal swab
Room temperature: 30 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received room temperature less than 14 days • Received refrigerated or frozen • Hemolysis
Methodology
Polymerase Chain Reaction • Direct DNA Sequencing
Setup Schedule
Set up: Mon-Sat a.m.; Report available: 7-9 days
Reference Range
See Laboratory Report
Performing Laboratory
Versiti Wisconsin, Inc
638 N 18 St
Milwaukee, WI 53233-2121
