Coxiella burnetii DNA, Qualitative Real-Time PCR

Test Code

16596

Preferred Specimen

0.7 mL serum or CSF or bronchial alveolar lavage (BAL) collected in a sterile container

Minimum Volume

0.3 mL

Other Acceptable Specimens

Whole blood collected in: a EDTA (lavender-top), or acid citrate dextrose ACD (yellow-top) tube • Tissue collected in a sterile container

Instructions

Tissue should be frozen on dry ice and transported frozen via overnight express. All other specimens should be transported refrigerated (2–8°C) via overnight express.

Transport Temperature

Serum, CSF, bronchial lavage (BAL), whole Blood: Refrigerated (cold packs)
Tissue: Frozen

Specimen Stability

Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days

Methodology
Real-Time Polymerase Chain Reaction

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Mon-Sat; Report available: 2-3 days

Reference Range

Not detected

Clinical Significance

Real-Time PCR detection of Coxiella burnetii can be used as a tool in the diagnosis of both acute and chronic Q fever. Real-Time PCR assays offer superior sensitivity, rapid test result turn-around, and the ability to detect C. burnetii DNA in multiple specimen types.

Performing Laboratory

Quest Diagnostics Nichols Institute-San Juan Capistrano, CA

33608 Ortega Highway

San Juan Capistrano, CA 92675-2042

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.