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Quantitative HIV-1 Real-Time PCR
Test Code16290
Preferred Specimen
2.2 mL plasma collected in an EDTA (lavender-top) tube
Minimum Volume
1.1 mL
Other Acceptable Specimens
Plasma collected in: PPT-potassium EDTA (white-top), or ACD solution A (yellow-top)
Instructions
Separate plasma from cells by centrifugation within 6 hours of collection and transfer the plasma to a screw-capped vial, freeze, and ship frozen.
Transport Container
Plastic screw-cap vial
Transport Temperature
Frozen
Specimen Stability
Room temperature: 24 hours
Refrigerated: 5 days
Frozen: 35 days
Refrigerated: 5 days
Frozen: 35 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Frozen plasma received in PPT (in situ)
Methodology
Real-Time Polymerase Chain Reaction (RT-PCR)
Setup Schedule
Set up: Tues, Fri; Report available: Next day
Reference Range
| <40 | copies/mL |
| <1.60 | Log copies/mL |
Clinical Significance
This test is intended for the use in conjunction with clinical presentation and other laboratory markers of disease progress for the clinical management of HIV-1 infected patients. The test can be used to assess patient prognosis by measuring the baseline HIV-1 RNA level or to monitor the effects of antiretroviral therapy by measuring changes in EDTA plasma HIV-1 RNA levels during the course of antiretroviral treatment.
Performing Laboratory
| Quest Diagnostics Nichols Institute |
| 14225 Newbrook Drive |
| Chantilly, VA 20153 |
