Hemoglobin, Free, Plasma

Test Code

514

Preferred Specimen

1 mL plasma collected in a sodium heparin (green-top) tube

Minimum Volume

0.2 mL

Other Acceptable Specimens

Lithium heparin (green-top) tube

Instructions

Centrifuge plasma within 1 hour of collection. Transfer the plasma to a sterile, plastic, screw-cap vial.

Transport Container

Plastic screw-cap vial

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 7 days
Refrigerated: 7 days
Frozen: Unacceptable

Methodology
Colorimetry (C)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Tues-Sat; Report available: 1-2 days

Reference Range

<8.4 mg/dL

Clinical Significance

The presence of free hemoglobin in plasma is an indication of intravascular hemolysis resulting from numerousconditions, among which include Paroxysmal Nocturnal Hemoglobinuria (PNH), Sickle-Cell Disease (SCD), Thalassemias, Hereditary Spherocytosis, Microangiopathic Hemolytic Anemias, Pyruvate Kinase Deficiency, ABO Mismatch Transfusion Reaction, Cardiopulmonary Bypass or Mechanical Heart Valve-induced Anemia.
Reference: JAMA. 2005;293(13):1653-1662

Performing Laboratory

Quest Diagnostics Nichols Institute

14225 Newbrook Drive

Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.