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T-Cell Subsets
MessagePerformed in the Hematology/Flow Cytometry Laboratory x5479.
Can be combined with purple top (EDTA) from routine or special hematology.
Due to 24 hour sample stability, samples cannot be collected on Saturdays or Sunday of a 3-day weekend.
Can be combined with purple top (EDTA) from routine or special hematology.
Due to 24 hour sample stability, samples cannot be collected on Saturdays or Sunday of a 3-day weekend.
Test Code
TCELL
CPT Codes
86359, 86360
Includes
CD 3, CD 4 and 8 % AND absolute counts. CD4:8 ratio.
CD19, 20, and 56 upon separate request
CD19, 20, and 56 upon separate request
Preferred Specimen
whole blood
Minimum Volume
0.5 mL
Instructions
Store samples at Room Temperature. DO NOT refrigerate
Transport Container
3 mL Purple Top (EDTA)
Transport Temperature
Room Temperature
Specimen Stability
Room Temperature: 24 hours
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis, QNS, Clotted
Methodology
Flow Cytometry
Setup Schedule
M-F 9AM-7PM
Limitations
Abnormal states of health are not always represented by abnormal percentages of certain leukocyte percentages as in a healthy person. Use test results in conjunction with clinical and other diagnostic data.
Reference Range
for male/females age 0-150 years: CD3 absolute: age 0-9 days: 1.6-5.00
CD3 %: 60-90 age 9 days-1 yr: 2.0-6.50
CD4 %: 30-65 age 1-2 yr: 1.4-5.40
CD8 %: 15-50 age 2-5 yr: 1.4-4.30
CD4:8 ratio: 0.7-3.5 age 5-8 yr: 1.0-3.40
CD4 absolute: 0.550-1.65 K/uL > 8 year: 1.0-2.50
CD8 absolute: 0.3-1.00 K/uL
CD3 %: 60-90 age 9 days-1 yr: 2.0-6.50
CD4 %: 30-65 age 1-2 yr: 1.4-5.40
CD8 %: 15-50 age 2-5 yr: 1.4-4.30
CD4:8 ratio: 0.7-3.5 age 5-8 yr: 1.0-3.40
CD4 absolute: 0.550-1.65 K/uL > 8 year: 1.0-2.50
CD8 absolute: 0.3-1.00 K/uL
Clinical Significance
T Helper and Suppressor cells enumeration in HIV positive patients
CD3+, CD4+, and CD8+ lymphocyte percentages and absolute counts may be used as aids to evaluate immune competency, underlying known or unknown disease states, and to monitor levels following organ transplantation.
CD3+, CD4+, and CD8+ lymphocyte percentages and absolute counts may be used as aids to evaluate immune competency, underlying known or unknown disease states, and to monitor levels following organ transplantation.

