T-Cell Subsets 

Message
Performed in the Hematology/Flow Cytometry Laboratory  x5479.
Can be combined with purple top (EDTA)  from routine or special hematology. 
Due to 24 hour sample stability, samples cannot be collected on Saturdays or Sunday of a 3-day weekend.


Test Code
TCELL


CPT Codes
86359, 86360

Includes
CD 3, CD 4 and 8 % AND absolute counts. CD4:8 ratio.
CD19, 20,  and 56 upon separate request 


Preferred Specimen
whole blood


Minimum Volume
0.5 mL


Instructions


Store samples at Room Temperature.  DO NOT refrigerate




Transport Container
3 mL Purple Top (EDTA)


Transport Temperature
Room Temperature


Specimen Stability
Room Temperature: 24 hours


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis, QNS, Clotted


Methodology
Flow Cytometry

Setup Schedule
M-F 9AM-7PM


Limitations


Abnormal states of health are not always represented by abnormal percentages of certain leukocyte percentages as in a healthy person. Use test results in conjunction with clinical and other diagnostic data.


  


Reference Range
for male/females age 0-150 years:             CD3 absolute:   age 0-9 days:  1.6-5.00  
CD3 %:  60-90                                                                      age 9 days-1 yr:  2.0-6.50
CD4 %:  30-65                                                                      age 1-2 yr:  1.4-5.40
CD8 %: 15-50                                                                       age 2-5 yr:  1.4-4.30
CD4:8 ratio:  0.7-3.5                                                              age 5-8 yr:  1.0-3.40
CD4 absolute:  0.550-1.65  K/uL                                           > 8 year:  1.0-2.50
CD8 absolute:  0.3-1.00  K/uL


Clinical Significance
T Helper and Suppressor cells enumeration in HIV positive patients
CD3+, CD4+, and CD8+  lymphocyte percentages and absolute counts may be used as aids to evaluate immune competency,  underlying known or unknown disease states,  and to monitor levels following organ transplantation.




The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.