| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Sickle Cell test
MessagePerformed in the Hematology Laboratory x5475, 76
Test Code
SICKS
CPT Codes
85660
Preferred Specimen
whole blood
Minimum Volume
1 mL
Instructions
Other Hematology tests requiring a Purple Top (EDTA) tube can
also be performed on this sample. If other tests are ordered,
a minimum of 3 mL is required.
also be performed on this sample. If other tests are ordered,
a minimum of 3 mL is required.
Transport Container
3 mL Purple Top (EDTA)
Transport Temperature
Room Temperature
Specimen Stability
Can be stored at 2-8C for up to 2 weeks.
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis, lipemia, QNS, clotted
Methodology
Modified Nalbandian solubility procedure
Setup Schedule
24/7
Limitations
This test cannot distinguish between Sickle cell trait and Sickle cell anemia.
This test will also be positive in the presence of other sickling hemoglobins
such as C Harlem. Therefore, if this test is positive a Hemoglobinopathy
Screen will be reflexively ordered for identification.
Elevated levels of hemoglobin F may cause false positive results
Samples from infants < 6 months of age may yield false negative results due to incomplete synthesis of hemoglobins at that age.
Samples from recently transfused patients may yield false negative results due to dilution with non-Hgb S transfused red cells.
This test will also be positive in the presence of other sickling hemoglobins
such as C Harlem. Therefore, if this test is positive a Hemoglobinopathy
Screen will be reflexively ordered for identification.
Elevated levels of hemoglobin F may cause false positive results
Samples from infants < 6 months of age may yield false negative results due to incomplete synthesis of hemoglobins at that age.
Samples from recently transfused patients may yield false negative results due to dilution with non-Hgb S transfused red cells.
Reference Range
Negative
Clinical Significance
Screening test for sickling hemoglobins (Primarily Hgb S). When test results are positive, a Hemoglobinopathy Screen is automatically ordered.
