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Anti SCL-70
MessagePerformed in the Chemistry Laboratory, Tufts Medical Center X5820
Test Code
SCL70
CPT Codes
86235
Preferred Specimen
Serum, Gold top
Acceptable: Red top
Acceptable: Red top
Minimum Volume
0.5 mL
Transport Container
Gold Top (SST)
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 8 hours
Refrigerated: 5 days
Frozen: One year
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Grossly lipemic, hemolyzed, or microbially specimens should not be used.
Methodology
Quantitative indirect immunofluorescence on the Phadia 250 (ThermoFisher Corp.)
Setup Schedule
This assay is performed twice per week.
Reference Range
<7.0 IU/mL (negative)
7.0-10.0 IU/mL (equivocal)
>10.0 IU/mL (positive)
Negative is considered normal. Reference values apply to all ages.
7.0-10.0 IU/mL (equivocal)
>10.0 IU/mL (positive)
Negative is considered normal. Reference values apply to all ages.
Clinical Significance
The determination of antinuclear antibodies (ANA) is of central importance for the clinical diagnosis of connective tissue diseases. Scl-70 antibodies are characteristic and specific for scleroderma (particularly the diffuse form; frequency up to 70%). Scleroderma is a group of autoimmune diseases that may result in changes to the skin, blood vessels, muscles, and internal organs. The disease can be either localized to the skin or involve other organs in addition to the skin. Symptoms may include areas of thickened skin, stiffness, feeling tired, and poor blood flow to the fingers or toes with cold exposure. One form of the condition, known as CREST syndrome, classically results in calcium deposits, Raynaud's syndrome, esophageal problems, thickening of the skin of the fingers and toes, and areas of small dilated blood vessels.
Performing Laboratory
Chemistry Laboratory, Tufts Medical Center X5820
