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Anti RNP/SM
MessagePerformed in the Chemistry Laboratory, Tufts Medical Center X5820
Test Code
RNP
CPT Codes
86235
Preferred Specimen
Serum, Gold top
Acceptable: Red top
Acceptable: Red top
Minimum Volume
0.5 mL
Transport Container
Gold Top (SST)
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 8 hours
Refrigerated: 5 days
Frozen: One year
Refrigerated: 5 days
Frozen: One year
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Grossly lipemic, hemolyzed, or microbially specimens should not be used.
Methodology
Quantitative indirect immunofluorescence on the Phadia 250 (ThermoFisher Corp.)
Setup Schedule
This assay is performed twice per week.
Reference Range
<5.0 IU/mL (negative)
5.0-10.0 IU/mL (equivocal)
>10.0 IU/mL (positive)
Negative is considered normal. Reference values apply to all ages.
5.0-10.0 IU/mL (equivocal)
>10.0 IU/mL (positive)
Negative is considered normal. Reference values apply to all ages.
Clinical Significance
The determination of antinuclear antibodies (ANA) is of central importance for the clinical diagnosis of connective tissue diseases. U1-snRNP/RNP70 antibodies typically appear in both SLE and mixed connective tissue disease (MCTD, Sharp Syndrome). MCTD is characterized by features of multiple autoimmune disorders, including systemic lupus erythematosus, systemic sclerosis, and polymyositis. Raynaud syndrome, joint pains, various skin abnormalities, and muscle weakness. This specific autoantibody is also sometimes present in these other autoimmune diseases. MCTD was characterized as an individual disease in 1972 by Sharp et al. and the term was introduced by Leroy in 1980.
Performing Laboratory
Tufts Medical Center Chemistry Laboratory X5820
