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Acid Elution
MessagePerformed in the Hematology Laboratory x5475, 76.
Test Code
ACEL
Alias/See Also
Kleihauer-Betke test
CPT Codes
85460
Preferred Specimen
whole blood
Minimum Volume
1 mL
Instructions
Can be performed with other Hematology assays using the same 4 mL Purple Top (EDTA) tube.
Transport Container
3 mL Purple Top (EDTA)
Transport Temperature
Room Temperature
Specimen Stability
24 hours at 2-8°C
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Grossly hemolyzed, clotted
Methodology
Hgb F stain
Setup Schedule
Results available daily on day shift by 11 AM. If the sample is received after 9 am, the test will be performed the following day by 11 AM.
Limitations
Patients with HPFH give false positive results
Reference Range
< 4 non-eluted cells/1000 RBC
Clinical Significance
Evalutate Fetal-maternal hemorrhage. Determines the number of fetal (hemoglobin F) Red cells in the maternal circulation.
In an Rh negative mother, this test is used to evaluate the amount of RhoGam to be administered to prevent possible Rh antibody formation.
In an Rh negative mother, this test is used to evaluate the amount of RhoGam to be administered to prevent possible Rh antibody formation.
