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HIV-1 RNA, Quantitative, Real-Time PCR
MessagePerformed in the Chemistry Laboratory, x5820
06.2018
06.2018
Test Code
HIV1
Alias/See Also
HIV RNA, PCR
CPT Codes
87536
Preferred Specimen
Plasma from a EDTA tube
Minimum Volume
10 mL whole blood EDTA
Instructions
Centrifuge within 6 hours of collection
Transport Container
Lavender Top (EDTA); Cryogenic Vial (5mL), frozen or dry ice
Transport Temperature
Room temperature for whole blood, Frozen or dry ice for plasma
Specimen Stability
20 - 25 °C: 24 hours
2 - 8 °C: 5 days
– 20 °C: 60 days
2 - 8 °C: 5 days
– 20 °C: 60 days
Methodology
Real-Time Transcription-Mediated Amplification (TMA)
Setup Schedule
This assay is performed 2x per week (Monday and Thursday)
Reference Range
Not Detected.
The test in use at Tufts Medical Center (Hologic Panther) can accurately quantify as low as 30 copies/mL.
The test in use at Tufts Medical Center (Hologic Panther) can accurately quantify as low as 30 copies/mL.
Clinical Significance
This test is used to assess prognosis and to monitor the efficacy of antiretroviral therapy.
