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Crossmatch (Flow Cytometry)
MessagePerformed by the HLA Molecular Pathology Laboratory. x5791 or x5793
This test is performed as the preliminary and final crossmatch between the recipient and chosen donor.
This test is performed as the preliminary and final crossmatch between the recipient and chosen donor.
Test Code
X-AlloFXM-Pre / X-AlloFXM-Final
CPT Codes
86825
Includes
Crossmatch for T and B cells
Preferred Specimen
Whole blood and serum
Minimum Volume
Collection vials (see below) must be full.
Instructions
Phlebotomist must initial tube and requisition for positive identification of both the recipient and donor(s)
Transport Container
6, 10mL Yellow tops (ACD) and 2, 10mL Red tops
Transport Temperature
Room Temperature
Specimen Stability
Room Temperature: 48 hours
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis, QNS, Clotted
Methodology
Flow cytometric analysis of fluorescent antigen/antibody complexes
Setup Schedule
M-F 8-4pm, as scheduled by Renal Transplant Clinic
Report Available
In Presidio (Teleresult)
Limitations
Increased sensitivity may result in the detection of weak antibodies.
Reference Range
Negative
Clinical Significance
To assess compatibility of donor and recipient for solid organ transplantation.
