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KOH PREP
MessagePerformed in the Microbiology Laboratory X65785
Test Code
KOH
Alias/See Also
Fungus Smear
CPT Codes
87220 (skin hair nails) 87210 (Tissue)
Includes
Microscopic examination for yeast/fungal elements
Preferred Specimen
Tissue biopsy
Skin scraping
Hair
Nails
Skin scraping
Hair
Nails
Instructions
Skin: Disinfect surface with 70% alcohol. Collect specimen from the edge of the lesion. Place in dry urine cup or tube or may inoculate medium directly (obtained from lab), or place between 2 glass slides taped together and placed in slide carrier or urine cup.
Hair: Scrape scalp and collect 10 broken hairs.
Nail: Scrap infected nail bed area or clip infected nail. Scalp: scrape with sterile toothbrush or small hairbrush.
Tissue: Collect approximately pea size specimen. Transport with just enough nonbacteriostatic saline to prevent drying.
Hair: Scrape scalp and collect 10 broken hairs.
Nail: Scrap infected nail bed area or clip infected nail. Scalp: scrape with sterile toothbrush or small hairbrush.
Tissue: Collect approximately pea size specimen. Transport with just enough nonbacteriostatic saline to prevent drying.
Transport Container
Sterile, screw cap container
Transport Temperature
Room Temperature
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Will not be performed on swab specimens or directly inoculated slides from ocular lesions.
Methodology
Microscopic exam
Setup Schedule
Test Performed Monday through Friday 6am-5pm
Limitations
KOH is not recommended as a screen for Yeast (Candia) or Fungal elements without a fungal culture, Culture,Fungal CXFUN or Culture, Fungal Skin CXFNS
Reference Range
Negative
Performing Laboratory
Microbiology X65785/ X65786

