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CD34
Message
Performed in the Hematology/Flow Cytometry Laboratory x5479.
Deliver to lab immediately after collection or processing.
Test takes 2.5-3 hours to perform, therefore cannot be done STAT
Test Code
N/A
CPT Codes
86367
Preferred Specimen
whole blood or apheresis product
Minimum Volume
1 mL
Transport Container
4 mL Purple Top (EDTA)
Transport Temperature
Room Temperature
Specimen Stability
Store specimens at room temperature (18 – 25oC), and process within 24 hours of collection.
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Grossly hemolyzed, QNS, or clotted
Methodology
Flow cytometry Stem Kit using CD34 antibodies
Setup Schedule
M-F only 9AM- 6PM.
After 5 pm and on weekends, by prior arrangement only.
Call 6-5479 Flow Cytometry Lab.
After 5 pm and on weekends, by prior arrangement only.
Call 6-5479 Flow Cytometry Lab.
Limitations
The presence of nucleated red blood cells, abnormal protein concentration, or hemoglobinopathies may result in unlysed red blood cells being counted as leukocytes, and may cause falsely decreased CD34% results. The CD34+absolute counts should not be affected.
Reference Range
Reference range for normal whole blood:
CD34+ (cells/μL): 0.50 – 6.50
CD34+ (%): 0.015 – 0.600
Clinical Significance
Quantitate HPC (Human Progenitor Cells) in peripheral blood to predict ideal harvest time.
To quantitate HPC collected in apheresis product after harvest, to determine if further collections are needed.
To quantitate HPC in products for infusion (transplantation)
To quantitate HPC collected in apheresis product after harvest, to determine if further collections are needed.
To quantitate HPC in products for infusion (transplantation)
