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Culture, Sputum, CF (Cystic Fibrosis) Protocol
MessagePerformed in the Microbiology Laboratory x5785
Selective media used for Burkholderia and Staphylococcus aureus isolation in addition to routine sputum protocol.
Gram stain will be performed on sputum samples only (not on deep throat swabs).
Selective media used for Burkholderia and Staphylococcus aureus isolation in addition to routine sputum protocol.
Gram stain will be performed on sputum samples only (not on deep throat swabs).
Test Code
CXCF
Alias/See Also
Culture, CF protocol
CPT Codes
87070, 87205
Includes
Bacterial culture (CPT 87070) and Gram stain (CPT 87205).
(Reflex to additional bacterial isolate identification if pathogenic organisms are isolated CPT 87077 and bacterial suceptibility if required)
(Reflex to additional bacterial isolate identification if pathogenic organisms are isolated CPT 87077 and bacterial suceptibility if required)
Preferred Specimen
Sputum or deep throat swab
Instructions
Expectorated sputum: Have patient rinse or gargle with water and then to cough deeply to produce lower respiratory tract specimen (not postnasal fluid or saliva).
Induced specimen: have patient rinse mouth with water after brushing gums and tongue. With aid of nebulizer, have the patient inhale about 25 ml of 3-10% normal saline. Collect via suction for pediatric patient unable to produce specimen.
Deep Throat: If sputum specimen not able to be obtained, obtain deep Throat specimen. Using a tongue blade to hold the tongue down, sample back of throat and tonsillar area. Do not touch the cheeks, teeth, or gums with the swab as you withdraw it from the mouth. Send in transport medium.
Induced specimen: have patient rinse mouth with water after brushing gums and tongue. With aid of nebulizer, have the patient inhale about 25 ml of 3-10% normal saline. Collect via suction for pediatric patient unable to produce specimen.
Deep Throat: If sputum specimen not able to be obtained, obtain deep Throat specimen. Using a tongue blade to hold the tongue down, sample back of throat and tonsillar area. Do not touch the cheeks, teeth, or gums with the swab as you withdraw it from the mouth. Send in transport medium.
Transport Container
Sterile, screw cap cup or Throat Culture Swab
Transport Temperature
Room Temperature
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimens are NOT rejected by gram stain criteria as in routine sputum protocol.
Methodology
Culture
Setup Schedule
Test performed Sunday through Saturday 6am- 11pm
Performing Laboratory
Microbiology X65785 / X65786
