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Cerebrospinal Fluid (CSF) Culture (spinal tap)
MessagePerformed in the Microbiology Laboratory x5785
Minimum volume 1 mL for bacterial culture; more if combining with other requests.
Indicate on requisition is specimen is from shunt collection.
Minimum volume 1 mL for bacterial culture; more if combining with other requests.
Indicate on requisition is specimen is from shunt collection.
Test Code
CXCSF
Alias/See Also
Culture, CSF
CPT Codes
87070, 87205
Includes
Bacterial culture (CPT 87070) and Gram stain (CPT 87205)
Preferred Specimen
Tube #2 or most turbid tube. Minimum volume 1 mL for bacterial culture; more if combining with other requests.
Specimens from shunt should be sent in capped syringe.
Specimens from shunt should be sent in capped syringe.
Minimum Volume
1mL for bacterial culture and gram stain.
Greater volume required if additional tests are requested.
Greater volume required if additional tests are requested.
Instructions
Decontaminate skin. Insert needle with stylet at L3-L4, L4-L5 or L5-S1 interspace.
Upon reaching subarachnoid space, remove sytlet, and collect 1 to 2 ml of fluid into each of 3 to 4 (depending on studies required) leakproof tubes.
Upon reaching subarachnoid space, remove sytlet, and collect 1 to 2 ml of fluid into each of 3 to 4 (depending on studies required) leakproof tubes.
Transport Container
Sterile, screw cap tube
Transport Temperature
Room temperature
Methodology
Culture
Setup Schedule
Test performed Sunday through Saturday 6am- 11pm
Reference Range
No growth
Performing Laboratory
Microbiology X65785 / x65786

