Culture, Acanthamoeba

Message
Cornea scraping or Corneal Biopsy or  Contact Lens
Call Microbiology Laboratory  prior to collection of specimen to alert them of procedure (617-636-5785).
Requires special media preparation- Page's Amoeba Saline- for processing.


Test Code
CXACN


Alias/See Also
Acanthamoeba Culture


CPT Codes
87177

Preferred Specimen
Corneal scraping or biopsy submitted in Page's Amoeba Saline


Other Acceptable Specimens
Contact lens submitted in Page's Amoeba saline.


Instructions
Using sterile spatula, scrape advancing edge of ulcer or lesion.
Inoculate scraping into Page's Amoeba saline tube.
Label tube with patient name and medical record number and/or date of birth.


Transport Container
Tube of 0.5 mL Page's Amoeba saline


Transport Temperature
Room Temperature




Specimen Stability
Transport to Microbiology 4-8 hours
>24 hours- rejected


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Specimens not submitted in Page's Amoeba saline.




Methodology
Culture

Setup Schedule
Test performed Sunday through Saturday 6am- 11pm


Reference Range
Negative


Performing Laboratory
Microbiology X 65785 / X65786



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.