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DNA Double-Stranded (dsDNA) Antibodies, IgG, Serum
MessagePerformed in the Chemistry Laboratory, Tufts Medical Center X5820
Test Code
ADNA
Alias/See Also
Mayo Test ID: ADNA
CPT Codes
86225
Preferred Specimen
Serum, Gold top
Acceptable: Red top
Minimum Volume
0.5 mL
Specimen Stability
Room temperature: 8 hours
Refrigerated: 5 days
Frozen: One year
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Grossly lipemic, hemolyzed, or microbially specimens should not be used.
Methodology
Quantitative indirect immunofluorescence on the Phadia 250 (ThermoFisher Corp.)
Setup Schedule
This assay is performed twice per week.
Reference Range
<30.0 IU/mL (negative)
30.0-75.0 IU/mL (borderline)
>75.0 IU/mL (positive)
Negative is considered normal. Reference values apply to all ages.
30.0-75.0 IU/mL (borderline)
>75.0 IU/mL (positive)
Negative is considered normal. Reference values apply to all ages.
Clinical Significance
IgG antibodies to double-stranded DNA (dsDNA) are a criterion for the diagnosis of systemic lupus erythematosus (SLE). These autontibodies are detectable in approximately 85% of patients with untreated SLE, and are rarely detectable at high titers in other connective tissue diseases. Weakly-positive results caused by low-avidity antibodies to dsDNA are not specific for SLE and can occur in a variety of diseases.
Testing for IgG antibodies to dsDNA is indicated in patients who have a positive test for antinuclear antibodies (ANA) along with signs and symptoms that are compatible with the diagnosis of SLE. If the ANA test is negative, there is no reason to test for antibodies to dsDNA.
The levels of IgG antibodies to dsDNA in serum are known to fluctuate with disease activity in lupus erythematosus, often increasing prior to an increase in inflammation and decreasing in response to therapy.
Testing for IgG antibodies to dsDNA is indicated in patients who have a positive test for antinuclear antibodies (ANA) along with signs and symptoms that are compatible with the diagnosis of SLE. If the ANA test is negative, there is no reason to test for antibodies to dsDNA.
The levels of IgG antibodies to dsDNA in serum are known to fluctuate with disease activity in lupus erythematosus, often increasing prior to an increase in inflammation and decreasing in response to therapy.
Performing Laboratory
Tufts Medical Center Chemistry Laboratory X5820
