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ANCA Confirmation (MPO and PR3)
MessageThis assay is used for pANCA confirmation and cANCA confirmation.
Performed in the Chemistry Laboratory, Tufts Medical Center X5820
Performed in the Chemistry Laboratory, Tufts Medical Center X5820
Test Code
ANCAC
CPT Codes
83516
Includes
MPO (Myeloperoxidase)
PR3 (Protease 3)
PR3 (Protease 3)
Preferred Specimen
Serum, Gold top
Acceptable: Red top
Minimum Volume
0.5 mL
Transport Container
Gold Top (SST)
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 8 hours
Refrigerated: 5 days
Frozen: One year
Methodology
Quantitative indirect immunofluorescence on the Phadia 250 (ThermoFisher Corp.)
Setup Schedule
This assay is performed twice per week.
Reference Range
MPO: <3.5 units –Negative
3.5 – 5.0 units - Equivocal
>5.0 units -Moderate to strong positive
PR3: <2.0 units –Negative
2.0 – 3.0 units - Equivocal
>3.0 units -Moderate to strong positive
3.5 – 5.0 units - Equivocal
>5.0 units -Moderate to strong positive
PR3: <2.0 units –Negative
2.0 – 3.0 units - Equivocal
>3.0 units -Moderate to strong positive
Clinical Significance
This test is used in conjunction with indirect immunofluorescence procedures to substantiate equivocal or positive pANCA or cANCA. The perinuclear staining pattern on neutrophils is most commonly associated with antibodies to myeloperoxidase. The cytoplasmic staining pattern on neutrophils is most commonly associated with antibodies to proteinase 3.
Performing Laboratory
Tufts Medical Center Chemistry Laboratory x5820.
