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HSV - Herpes Simplex Virus, Type 1 and 2 DNA, Quantitative, Real-Time PCR (CSF, Fluids, Blood)
Performed at Quest Diagnostics

Message
HSV PCR (CSF, Blood, Fluids - Pericardial, Pleural ) Qualitative Real-Time Polymerase Chain Reaction (PCR)

Clinical Significance
Diagnosis of active Herpes simplex infection.
 


Test Code
HSVQ


Alias/See Also
HSV PCR (CSF, Fluids, BLOOD)


CPT Codes
87530 (x2)

Preferred Specimen

1 mL CSF,  pericardial, pleural, amniotic fluid or serum collected in a sterile container.
Plasma or whole blood collected in an EDTA (lavender-top) or ACD (yellow-top) tube
 


Minimum Volume
1 mL Preferred (0.3mL minimum volume)


Other Acceptable Specimens
CSF, Pericardial Fluid, Pleural Fluid, Amniotic Fluid, (Serum. from a sterile container) Plasma or Whole Blood (EDTA or ACD ) tubes  


Instructions
Fluids in sterile collection containers.

Do not freeze whole blood


Transport Temperature
Transport Temperature: transport Specimen to laboratory As Soon As Possible 
 
Transport to reference lab :
Whole blood: Refrigerated (cold packs) Do not freeze whole blood

All other specimen types: Frozen
 


Specimen Stability
Specimen Stability
Whole blood- Room temperature: 48 hours, Refrigerated: 7 days, Frozen: Unacceptable

All other specimen types, Room temperature: 48 hours, Refrigerated: 7 days, Frozen: 30 days
 


Methodology
Real time  Polymerase Chain Reaction
The detection of HSV-1 and HSV-2 DNA is based upon the real-time amplification, detection and differentiation of specific HSV-1 and HSV-2 genomic DNA sequences by PCR from total DNA extracted from the specimen.
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
 

Setup Schedule
Reference Lab - Quest- Daily


Report Available
Set up: Daily; Report available: 2 days
 


Reference Range
Real-Time Polymerase Chain Reaction (PCR)
Reference Range(s)
HSV 1 DNA Not detected
HSV 2 DNA Not detected


Clinical Significance
Clinical Significance
Diagnosis of active Herpes simplex infection.
The detection of HSV-1 and HSV-2 DNA is based upon the real-time amplification, detection and differentiation of specific HSV-1 and HSV-2 genomic DNA sequences by PCR from total DNA extracted from the specimen


Performing Laboratory
Performing Laboratory
Quest Diagnostics Nichols Institute-Chantilly VA
14225 Newbrook Drive
Chantilly, VA 20151-2228




The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.