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Influenza A and B PCR
MessageIn "Flu" season an Influenza PCR test will be performed first to r/o Influenza A and B. If patient is immunocompromised, critically ill due to respiratory infection or a pediatric patient at high risk for severe complications due to cardiopulmonary disease, consider RVP panel. Call 65785 Microbiology. For Mycoplasma, Chlamydophilia or Bordetella pertussis testing order APNEU Atypical pneumonia panel
Test Code
FLUPR
Alias/See Also
FLU PCR,
CPT Codes
87502
Includes
Influenza A & B by PCR, Report differentiates between Influenza A and influenza B.
Preferred Specimen
Nasopharyngeal swab (obtain collection kit from Microbiology lab, 6-5785)
Instructions
Deep nasopharyngeal (not nasal) collection is important to test sensitivity.
Insert swab through the nose into the posterior nasopharynx and rotate the swab. Place the inoculated swab into the Viral Transport Media and break off the shaft where scored. Fit the top of the shaft into the lid of the tube and screw top on securely.
Insert swab through the nose into the posterior nasopharynx and rotate the swab. Place the inoculated swab into the Viral Transport Media and break off the shaft where scored. Fit the top of the shaft into the lid of the tube and screw top on securely.
Transport Container
M6 Viral Transport Media available from Microbiology x65785
Transport Temperature
Room Temperature if sent promptly Refrigerated if transport will be delayed beyond 4 hours
Specimen Stability
room temperature 4 hours
Refrigerated 1 week
Refrigerated 1 week
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Non-nasopharnygeal specimens, nasal wash, routine bacterial swab , sputum
Specimens not collected in M6 viral transport media
Specimens not collected in M6 viral transport media
Methodology
RT-PCR
Setup Schedule
"Flu" season Sunday to Satuday 24 hours /day.
This test is not performed outside of Flu season due to low levels of circulating Influenza in the community. See RVP (Respiratory Viral pathogens) test
This test is not performed outside of Flu season due to low levels of circulating Influenza in the community. See RVP (Respiratory Viral pathogens) test
Report Available
Within 2 hours of recipt in the lab.
Limitations
Nucleic acids may persist in vivo independent of organism viability. Therefore, detection of organism target(s) does not imply that the corresponding organisms are infectious or are the causative agents for clinical symptoms. Results must be correlated with the clinical history, epidemiological data, and other data available to the clinician evaluating the patient.
Reference Range
Not performed
Performing Laboratory
Microbiology x65785
