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Herpes Simplex Virus, Type 1 and 2 DNA, Qualitative, Real-Time PCR - (CSF, mucocutaneous lesions or vesicles)
MessageReplaces HSV culture and HSV DFA for CSF, muco-cutaneous lesions since 2019. Viral culture still recommended if Varicella zoster virus (chicken pox or shingles) is suspected CXVIR
Test Code
HSVNA
Alias/See Also
HSV PCR, Herpes Simplex PCR, HSV 1 and 2 Qualitative Assay
CPT Codes
87529 (HSV amplified probe technique)
Includes
HSV PCR : Amplified detection of Herpes Simplex Virus and strain identification HSV 1 or HSV 2
Preferred Specimen
CSF, (in sterile tube) Mucu-cutaneous lesion or vesicle contents in viral transport media (call Microbiology lab x65785 for media. Genital, oral, eye, skin, rectal lesions and vesicles.
Minimum Volume
CSF (0.2 ML )
Instructions
Fresh Vesicle: Cleanse area with sterile saline. Carefully open vesicle with a needle or scalpel blade. With swab, collect fluid and cellular material by vigorously sampling base of lesion . Place into Viral Transport Media.
Aspirated fluid or lesion scraping. Aspirate fluid from vesicle with 26- or 27- gauge needle attached to tuberculin syringe and rinse into 1-2 mL of VTM. Follow with swab back and forth over lesion with rayon swab in viral transport kit, and place swab into same VTM with aspirated fluid.
Cervix: Remove ectocervical mucus with a swab and discard swab. incert swab form viral transport kit at lest 1cm into cervical cana and rotate swab for 10 seconds. Place into Viral Transport Media. Urethra: Express and discard any exudate. Insert flexible fine -shafted swab 2-3 com into urethra, gently rotate swab two to three times and withdraw. Place into Viral Transport Media.
Rectal: Carefully insert swab beyond anal sphincter and gently rotate to sample anal crypts. Place into Viral Transport Media.
CSF Sterile Tube.
Aspirated fluid or lesion scraping. Aspirate fluid from vesicle with 26- or 27- gauge needle attached to tuberculin syringe and rinse into 1-2 mL of VTM. Follow with swab back and forth over lesion with rayon swab in viral transport kit, and place swab into same VTM with aspirated fluid.
Cervix: Remove ectocervical mucus with a swab and discard swab. incert swab form viral transport kit at lest 1cm into cervical cana and rotate swab for 10 seconds. Place into Viral Transport Media. Urethra: Express and discard any exudate. Insert flexible fine -shafted swab 2-3 com into urethra, gently rotate swab two to three times and withdraw. Place into Viral Transport Media.
Rectal: Carefully insert swab beyond anal sphincter and gently rotate to sample anal crypts. Place into Viral Transport Media.
CSF Sterile Tube.
Transport Container
Viral transport media from Microbiology laboratory x65785
Transport Temperature
Room temperature if sent to lab immediately if transport > 15 minutes please send on ice
Specimen Stability
15 minutes at room temperature
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Non -lesion or on -vesicle sources. Resiratory specimens
This test is not approved for these sources for HSVPCR (sent to Quest Diagnostics)
Other specimen types not approved for this method may be tested by HSV culture (CXHSV)
If sites are to be screened for HSV and Varicella Zoster Virus, VZV culture will be performed for VZV only if HSV PCR is negative. Indicate on requisition if both viruses may be suspected.
This test is not approved for these sources for HSVPCR (sent to Quest Diagnostics)
Other specimen types not approved for this method may be tested by HSV culture (CXHSV)
If sites are to be screened for HSV and Varicella Zoster Virus, VZV culture will be performed for VZV only if HSV PCR is negative. Indicate on requisition if both viruses may be suspected.
Methodology
Amplified probe technique -PCR
FDA Status
Approved
Setup Schedule
Sunday to Saturday Once daily
Limitations
Not suitable for Non lesion or non vesicle sources such as CSF, respiratory specimens.
Please note specific body site and viruses to rule out on requisition.
Viral culture will not be reflexed if HSV PCR is Negative.
Please note specific body site and viruses to rule out on requisition.
Viral culture will not be reflexed if HSV PCR is Negative.
Reference Range
Not Detected
Clinical Significance
Herpes Simplex Virus causes a multitude of human diseases and are particularly severe in patients whose immune systems are immature (neonates), suppressed (drug therapy) or compromised (elderly, AIDS).
HSV infections include genital lesions, cold sores, pharyngitis, ocular keratitis and encephalitis.
HSV infections include genital lesions, cold sores, pharyngitis, ocular keratitis and encephalitis.
Performing Laboratory
Microbiology-Virology laboratory x65785
