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Hemoglobin F quantitation
MessagePerformed in the Hematology Laboratory x5475, 76
Test Code
HBF
Alias/See Also
Hemoglobin F
Fetal hemoglobin
Fetal hemoglobin
CPT Codes
83030
Preferred Specimen
Whole blood
Minimum Volume
1 mL
Transport Container
3 mL Purple Top (EDTA)
Transport Temperature
Room Temperature
Specimen Stability
Stable refrigerated for up to 7 days.
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Grossly hemolzed, QNS, clotted
Methodology
HPLC
Setup Schedule
Tuesday and Fridays, 9-3 pm
Limitations
Hemoglobin F can be elevated in pregnancy.
Reference Range
0-3mo: 15-81%
3-6mo: 2.3- 56 %
6mo.-1yr: 0.8-4.9%
1-2 yrs: 0.7-2.1%
>2yrs: 0.4-2.0%
3-6mo: 2.3- 56 %
6mo.-1yr: 0.8-4.9%
1-2 yrs: 0.7-2.1%
>2yrs: 0.4-2.0%
Clinical Significance
Performed as part of the Hemoglobinopathy Screen.
Elevated in infancy, Sickle cell anemia, beta thalassemia, and in HPFH.
Elevated in infancy, Sickle cell anemia, beta thalassemia, and in HPFH.
