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Culture, Blood
MessageIf culture is positive, identification and antimicrobial susceptibility will be performed at an additional charge. Clinical specimens should be collected before the initiation of therapy since even a few days of treatment may render the culture negative. Day(s) Test Set Up: Monday through Sunday
Test Code
BCUL
CPT Codes
87040
Preferred Specimen
Pediatric - 1 aerobic bottle
Adult - 2 sets (1 aerobic & 1 anaerobic bottle per set)
Adult - 2 sets (1 aerobic & 1 anaerobic bottle per set)
Instructions
Bactec® blood culture bottles must be used for this procedure.
Pediatric: Draw a single specimen, unless otherwise specified, as follows: 1. Sterilize top of 1 Bactec blood culture bottle with alcohol. 2. Apply tourniquet to patient's arm. 3. Palpate area to locate vein. 4. Sterilize area with alcohol and and then a Chloraprep Frepp applicator or 2 iodines; let site dry for >1 minute. 5. Do not palpate vein after sterilizing area. 6. Draw blood using a Butterfly Collection Kit. 7. Place at least 1mL of blood and not more than 3mL of blood into the pediatric bottle. Mix bottle by inverting 4 or 5 times. 8. Label bottle with patient's full name, DOB, date, time of draw and the collection site. Adults- collect 2 sets of bottles from two different sites (no more than 3/24 hours), unless otherwise specified, as follows: 1. Sterilize tops of BacTec® blood culture bottles with alcohol. 2. Apply tourniquet to patient's arm. 3. Palpate area to locate vein. 4. Sterilize area with alcohol and then a Chloraprep Frepp applicator or 2 iodines; let site dry for >1 minute. 5. Do not palpate vein after sterilizing area. 6. Draw blood using a Butterfly Collection Kit. 7. Collect 5-10 mL of blood into the aerobic bottle and 5 -10 mL of blood into the anaerobic bottle. Mix the bottles by inverting 4 or 5 times. 8. Label the bottles with patient's full name, DOB, date, time of draw and the collection site. 9. Forward without delay to the laboratory.
Pediatric: Draw a single specimen, unless otherwise specified, as follows: 1. Sterilize top of 1 Bactec blood culture bottle with alcohol. 2. Apply tourniquet to patient's arm. 3. Palpate area to locate vein. 4. Sterilize area with alcohol and and then a Chloraprep Frepp applicator or 2 iodines; let site dry for >1 minute. 5. Do not palpate vein after sterilizing area. 6. Draw blood using a Butterfly Collection Kit. 7. Place at least 1mL of blood and not more than 3mL of blood into the pediatric bottle. Mix bottle by inverting 4 or 5 times. 8. Label bottle with patient's full name, DOB, date, time of draw and the collection site. Adults- collect 2 sets of bottles from two different sites (no more than 3/24 hours), unless otherwise specified, as follows: 1. Sterilize tops of BacTec® blood culture bottles with alcohol. 2. Apply tourniquet to patient's arm. 3. Palpate area to locate vein. 4. Sterilize area with alcohol and then a Chloraprep Frepp applicator or 2 iodines; let site dry for >1 minute. 5. Do not palpate vein after sterilizing area. 6. Draw blood using a Butterfly Collection Kit. 7. Collect 5-10 mL of blood into the aerobic bottle and 5 -10 mL of blood into the anaerobic bottle. Mix the bottles by inverting 4 or 5 times. 8. Label the bottles with patient's full name, DOB, date, time of draw and the collection site. 9. Forward without delay to the laboratory.
Transport Container
BD Bactec Blood Culture Bottles
Transport Temperature
Ambient
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Refrigerated, Overfilled, Underfilled bottles
Methodology
Bactec FX - Fluorochromic
Setup Schedule
Daily
Report Available
Preliminary report available after 24 hrs.
Reference Range
Negative
Clinical Significance
Useful for the detection of bacterial septicemia.
Last Updated: April 1, 2013
