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Monkeypox Virus DNA Qual RT PCR(Quest test code 12084)

Message
  • Consider submitting additional swabs if multiple lesions in different stages are present.
  • Multiple specimens collected on a single patient should be submitted separately and be accompanied by its own separate order and transported within its own sealed bag.
  • Outside facilities will complete a SRH Lab Requisition for EACH specimen submitted. 


Test Code
LAB12084


CPT Codes
87593(X2)

Preferred Specimen
Lesion Kit Swab in VCM (Viral Culture Media)


Minimum Volume
0.5 mL of VCM or equivalent


Other Acceptable Specimens
White-Top Culture Swab in VCM
Other acceptable transport media: VTM, UTM or equivalent


Instructions
  1. Using a lesion swab (or white-top culture swab), swab the pustule / lesion vigorously.
  2. Place the swab into viral culture media (VTM, UTM, or equivalent) tube.
  3. Label specimen at the bedside to ensure proper patient identification.
  4. Refrigerate sample IMMEDIATELY (Room Temp Unacceptable)
****If multiple specimens need to be collected for a single patient, they should be submitted separately and be accompanied by its own separate order and transported in its own sealed bag.


Transport Container
3mL of Viral Culture Media (VCM)
Other acceptable transport media: VTM, UTM or equivalent 


Transport Temperature
Refrigerate (or Freeze) sample immediately.
**Transport on Ice to SRH - Fill two ziploc bags with ice (check freezer for pre-filled bags). Place biohazard specimen bag that contains the specimen and requisition in a second biohazard bag. Place 2 ice-filled bags and specimen in the larger biohazard bag.

Room temperature is unacceptable - specimen will be rejected if received at room temperature.


Specimen Stability
Room Temperature: Unacceptable
Refrigerated: 7 days
Preferred-Frozen: 30 Days


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
  • Room Temperature
  • Dry Swab (not submitted in VCM or equivalent)
  • Cotton Swab, Wooden Shaft Swab, or Calcium Alginate Swabs


Methodology
Real-Time Polymerase Chain Reaction

Setup Schedule
Daily


Report Available
TAT: 2 - 3 days upon receipt of sample in Quest Laboratory.


Reference Range
Orthopoxvirus DNA, QL PCR - Not Detected
Monkeypox Virus DNA, QL PCR - Not Detected


Performing Laboratory
Quest Chantilly Diagnostics



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.