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Kappa/Lambda Light chains, Free with Ratio, Serum
MessageSRH Clients:
- 1 Yellow top tube or 1 Red top tube
- Transport to SRHC laboratory same day as collected
Test Code
FKLR
Alias/See Also
Lab7253
Free Kappa/Lambda with K/L Ratio
Free Kappa/Lambda with K/L Ratio
CPT Codes
83883 (x2)
Preferred Specimen
2 mL serum
Minimum Volume
1 mL serum
Transport Container
1 yellow top tube or 1 red top tube
Transport Temperature
Refrigerated
Specimen Stability
Refrigerated: 21 days
Frozen: 30 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis, Grossly lipemic, Microbially contaimined serum, specimens containing heavy visible particulate
Methodology
Immunoturbidimetric
Setup Schedule
Tuesday and Friday Mornings
Report Available
Tuesday and Friday
Reference Range
Kappa Light Chain, Free, Serum 3.3-19.4 mg/L
Lambda Light Chain, Free, Serum 5.7-26.3 mg/L
Kappa/Lambda Light Chains Free with Ratio, Serum 0.26-1.65
Lambda Light Chain, Free, Serum 5.7-26.3 mg/L
Kappa/Lambda Light Chains Free with Ratio, Serum 0.26-1.65
Clinical Significance
This test is a latex-enhances, immunoassay that provides ultrasensitive detection and quantitation of free light chains (FLCs) in serum earlier than electrophoresis. It is an aid in diagnosis and treatment of multiple myeloma, lymphocytic neoplasms, Waldenstroms macroglobulinemia and connective tissue disease, such as systemic lupus erythematosis.
Approximately 15% of all cases of multiple myeloma produce only free kappa or lambda light chains in excess. These patients frequently show no abnormality on serum protein electrophoresis. Quite often urine is tested for the presence of these light chains, which are also called Bence Jones Proteins. However, the FLCs entering the urine are strongly influenced by renal tubular function. When clonal proliferation of plasma cells starts to develop, the FLC concentration increases in serum. Since these small particles are rapidly cleared by the renal tubules, urine tests for FLCs remiain negative until the tumor mass expands, and the FLCs in serum exceed teh resorptive capacity of the renal tubules. Therefore, the measurement of FLCs in serum is an alternative to less sensitive urine testing. Changing concentrations of FLCs in serum relate better to changing tumor load than to concentrations in urine.
Approximately 15% of all cases of multiple myeloma produce only free kappa or lambda light chains in excess. These patients frequently show no abnormality on serum protein electrophoresis. Quite often urine is tested for the presence of these light chains, which are also called Bence Jones Proteins. However, the FLCs entering the urine are strongly influenced by renal tubular function. When clonal proliferation of plasma cells starts to develop, the FLC concentration increases in serum. Since these small particles are rapidly cleared by the renal tubules, urine tests for FLCs remiain negative until the tumor mass expands, and the FLCs in serum exceed teh resorptive capacity of the renal tubules. Therefore, the measurement of FLCs in serum is an alternative to less sensitive urine testing. Changing concentrations of FLCs in serum relate better to changing tumor load than to concentrations in urine.
Performing Laboratory
SRH Main lab
