C Difficile Toxin PCR reflex to GDH/Toxin (LAB1412301732)

Message

SRHC Clients:(Available beginning 09/10/2013)

Should not be performed on formed stool.
Transport to SRH lab in less than 24 hours room temp or refrigerated for best practice. REFRIGERATED IS PREFERRED.

Test Code

CDIFX

Alias/See Also

Clostridium difficile Toxin PCR/EPI PCR
C difficile toxin B Gene
C difficile 027-NAP1-B1
C diff pcr stool

CPT Codes
87493

Includes

C difficile Toxin B gene DNA detection
C difficile hypervirulent NAP-1 strain
Reflexes to C Difficile GDH Antigen/Toxins A&B

Preferred Specimen

Fresh liquid or soft stool in clean or sterile specimen container
Formed stools will not be tested

Transport Container

0.5 - 1 gram diarrheal stool in clean container

Transport Temperature

Room Temperature or Refrigerated--do not expose to excessive heat or freezing.
REFRIGERATED PREFERRED.

Specimen Stability

Room Temp 24 hours
Refrigerated 5 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Formed Stools
Collection on swabs or in diapers.
Specimens in formalin, PVA, Para-Pak C&S
Specimens contaminated with barium.
Lumen contents adn duodenal aspirates/washes/biopsies are not accepted.
Frozen specimen.
Specimen exceeding stability limits.

Methodology

Real-time Polymerase Chain Reaction(PCR)-Cepheid Gene Xpert

Setup Schedule

Daily

Report Available

Within 24 hours of receipt

Reference Range

C difficile toxin B Gene---negative(NEG)
C difficile 027-NAP1-B!--presumptive negative(PNEG)

Clinical Significance

027 NAP1 BI is a hypervirulent strain of c diff producing increased toxins and severe disease.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.