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Blood Culture
MessageSRHC Clients:
FOR COMPLETE PROCEDURE SEE INSTRUCTION SECTION BELOW:
NOTES:
Bottles available from SRH Lab-Bacteriology
Collect aseptically according to instructions.
Skin preparation and adequate volumes directly influence the results.
Best practice is to draw 2 to 3 blood culture sets from separate sites before starting antimicrobial therapy.
Drawing blood at specified intervals is only indicated when it is necessary to document continuous bacteremia in patients with suspected endocarditis.
Draw blood immediately prior to next dose if patient is on antibiotic therapy.
FOR COMPLETE PROCEDURE SEE INSTRUCTION SECTION BELOW:
NOTES:
Bottles available from SRH Lab-Bacteriology
Collect aseptically according to instructions.
Skin preparation and adequate volumes directly influence the results.
Best practice is to draw 2 to 3 blood culture sets from separate sites before starting antimicrobial therapy.
Drawing blood at specified intervals is only indicated when it is necessary to document continuous bacteremia in patients with suspected endocarditis.
Draw blood immediately prior to next dose if patient is on antibiotic therapy.
Test Code
BLDC
CPT Codes
87040
Includes
Culture with identification and sensitivity as indicated.
Preferred Specimen
ADULTS/CHILDREN 1 aerobic bottle(blue top) BacT/ALERT SA 10 mls
1 anaerobic bottle(purple top) BacT/ALERT SN 10 mls
NEONATES/INFANTS 1 pediatric bottle(yellow top) BacT/ALERT PF 4 mls
Minimum Volume
Adult/Children--If less than 4 mls is able to be obtained, PUT ALL OF THE SAMPLE IN THE BLUE AEROBIC BOTTLE.
Neonate/Infants--up to 4 mls in yellow pediatric bottle
Instructions
1. Select a different site for each blood culture set to be drawn.
2. Avoid drawing through IV or intra-arterial catheter. If blood cannot be drawn by routine venipuncture, it must be documented that IV or intra-arterial draw was approved by the physician.
3. Break the choloroprep ampule, taking care not to touch the sponge.(choloroprep cannot be used in infants under 2 months of age--use tincture of iodine or iodophor)
4. Press the sponge against the site to saturate the sponge.
5. Wet the venipuncture site completely using back and forth scrubbing motion for 30 seconds.
6. Allow the prepped area to dry completely. Do not blot or wipe the solution away.
7. Discard applicator after a single use.
8. Do not touch the site after it is prepped.
9. Remove the flip caps from the blood culture bottles. Mark the broth level and then mark 10 mL
10.Disinfect the top of the bottles with alcohol and allow to dry.
11.Connect the adapter cap to the luer connector of a butterfly set.
12. Perform venipunture. When the needle is in the vein, secure it with tape or hold in place.
13. On a flat surface, place adapter cap on the aerobic blue blood culture bottle septum and press down to penetrate and obtain blood flow
into the bottle.
14. Hold the adapter cap down on the bottle during the collection. Collect 10 mL per bottle - DO NOT overfill.
15. Line demarcations on the bottle indicate volume.
2. Avoid drawing through IV or intra-arterial catheter. If blood cannot be drawn by routine venipuncture, it must be documented that IV or intra-arterial draw was approved by the physician.
3. Break the choloroprep ampule, taking care not to touch the sponge.(choloroprep cannot be used in infants under 2 months of age--use tincture of iodine or iodophor)
4. Press the sponge against the site to saturate the sponge.
5. Wet the venipuncture site completely using back and forth scrubbing motion for 30 seconds.
6. Allow the prepped area to dry completely. Do not blot or wipe the solution away.
7. Discard applicator after a single use.
8. Do not touch the site after it is prepped.
9. Remove the flip caps from the blood culture bottles. Mark the broth level and then mark 10 mL
10.Disinfect the top of the bottles with alcohol and allow to dry.
11.Connect the adapter cap to the luer connector of a butterfly set.
12. Perform venipunture. When the needle is in the vein, secure it with tape or hold in place.
13. On a flat surface, place adapter cap on the aerobic blue blood culture bottle septum and press down to penetrate and obtain blood flow
into the bottle.
14. Hold the adapter cap down on the bottle during the collection. Collect 10 mL per bottle - DO NOT overfill.
15. Line demarcations on the bottle indicate volume.
Transport Container
Adult/children: 10 mL each in aerobic & anaerobic bottle (blue and purple top bottles). DO NOT EXCEED 10 ML PER BOTTLE.
If 4 ml or less is collected, put all of the sample in the aerobic blue bottle for adults/children
Pediatric: up to 4 mL in pedi bottle(yellow top)
If 4 ml or less is collected, put all of the sample in the aerobic blue bottle for adults/children
Pediatric: up to 4 mL in pedi bottle(yellow top)
Transport Temperature
Room Temperature
Specimen Stability
Transport to Lab ASAP so that processing can begin for faster results.
24 hours room temperature
24 hours room temperature
Methodology
Culture by BacT Alert automated system
Setup Schedule
24/7
Report Available
5 days if negative
Positives worked up as isolated
Positives worked up as isolated
