Serotonin Release Assay (SRA), Unfractionated Heparin

Message

This test was developed and its performance characteristics have been determined by Quest Diagnostics Nichols Institute. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. Performance characteristics refer to the analytical performance of the test.

SRHC Clients:

1 yellow top tube or 1 red top tube (no gel)
Must centrifuge to separate serum from cells and FREEZE as soon as possible
Transport FROZEN to SRHC Laboratory as soon as possible

Test Code

LAB7663

Quest Code

14627

Alias/See Also

EPIC: LAB7663

CPT Codes
86022

Preferred Specimen

1 mL serum (0.4 mL minimum)

Minimum Volume

0.4 mL

Instructions

Centrifuge and immediately separate serum specimens from the cells into clean, plastic, screw-capped vial(s). Freeze and ship frozen.

Transport Container

No additive red top tube

Transport Temperature

Frozen

Specimen Stability

Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 6 months

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Received room temperature • Received refrigerated

Methodology
Radiobinding Assay (RBA)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Sun-Fri; Report available: 1-2 days

Reference Range

See Laboratory Report

Clinical Significance

Any patient with an unexplained fall in the platelet count of >40% during or after cessation of heparin therapy should be suspected of hit-heparin induced thrombocytopenia. This test is a functional assay of the PFU-antibody complex with heparin.

Note: Patients should not be on ticagrelor (Brilinta) as this may result in a false negative serotonin release assay.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.