Lyme Disease Antibody with Reflex to Blot (IgG, IgM) - Quest 6646

Message

SRHC Clients:

1 gold top tube or 1 red top tube
Transmit to SRHC laboratory within 8 hours

Test Code

LAB788

Quest Code

6646

Alias/See Also

EPIC: LAB788
Borrelia burgdoferi

CPT Codes
86618

Includes

If Lyme Disease Antibody Screen is ≥0.90, then Lyme Disease Antibodies (IgG, IgM), Immunoblot will be performed at an additional charge (CPT code(s): 86617 x2).

Preferred Specimen

1mL serum (0.5 mL minimum)

Minimum Volume

0.7 mL

Transport Container

Transport tube

Transport Temperature

Refrigerate, stable 7 days

Specimen Stability

Room temperature: 7 days
Refrigerated: 14 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis
Gross lipemia

Methodology
Immunoassay (IA)

Setup Schedule

Set up: Tues-Sat; Report available: 1-3 days

Reference Range

Index	Interpretation
<0.90	Negative
0.90-1.09	Equivocal
>1.09	Positive

As recommended by the Food and Drug Administration (FDA), all samples with positive or equivocal results in a Borrelia burgdorferi antibody screen will be tested using a blot method. Positive or equivocal screening test results should not be interpreted as truly positive until verified as such using a supplemental assay (e.g., B. burgdorferi blot).
The screening test and/or blot for B. burgdorferi antibodies may be falsely negative in early stages of Lyme disease, including the period when erythema migrans is apparent.

Clinical Significance

Lyme disease is caused by a bacterium borrelia burgdorferi and is transmitted by ticks. A screening test with high sensitivity is used as the first step in the CDC recommended algorithm. Immunoblot testing qualitatively examines, with high specificity, antibodies in a patient's specimen. Immunoblot testing is appropriate for confirming a detected screening result.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.