Complement, Total (CH50)

Message

SRHC Clients:

1 yellow top tube or 1 red top tube (no gel)
Centrifuge and remove serum from cells within 1 hour of collection. Immediately freeze sample at -20 for transport
If unable to centrifuge and freeze sample, send patient to SRHC Laboratory for collection due to sample requirements.
Transport sample to SRHC laboratory FROZEN

Test Code

LAB154

Quest Code

618

Alias/See Also

EPIC: LAB154

CPT Codes
86162

Preferred Specimen

1 mL serum

Minimum Volume

0.5 mL

Instructions

Avoid hemolysis. Centrifuge serum specimens within 1 hour of collection. Immediately pipette serum into "sterile, plastic, screw-capped vial(s) and freeze solid" at -20°C or lower. Do not allow samples to thaw. With multiple tests, submit a separate tube for each test. Send frozen samples on dry ice to the laboratory. Do not submit the sample in a glass tube.

Transport Container

Transport tube

Transport Temperature

Frozen

Specimen Stability

Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 7 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis • Lipemia • Received thawed

Methodology
Liposome

Setup Schedule

Set up: Mon-Sat; Report available: 1-2 days

Reference Range

31-60 U/mL

Clinical Significance

CH50 is a screening test for total complement activity. Levels of complement may be depressed in genetic deficiency, liver disease, chronic glomerulonephritis, rheumatoid arthritis, hemolytic anemias, graft rejection, systemic lupus erythematosis, acute glomerulonephritis, subacute bacterial endocarditis and cryoglobulinemia. Elevated complement may be found in acute inflammatory conditions, leukemia, Hodgkin's Disease, sarcoma, and Behcet's Disease.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.