HLA-B27 Antigen

Message

SRHC Clients:

1 yellow top ACD-B tube or 1 yellow top ACD-A tube
1 lavender (EDTA ) or 1 dark green (Sodium heparin) tube also acceptable
DO NOT CENTRIFUGE
Transport to SRHC laboratory same day as collected at room temperature or refrigerated

Test Code

LAB869

Quest Code

528

Alias/See Also

Ankylosing Spondylitis

CPT Codes
86812<br><strong>This test code is for non-New York patient testing. For New York patient testing, use test code 93099.</strong>

Preferred Specimen

6 mL whole blood (1 mL minimum)

Minimum Volume

1 mL

Other Acceptable Specimens

Whole blood collected in: Acid citrate dextrose ACD-A or B (yellow-top), or EDTA (lavender-top) tube

Transport Temperature

Room temperature or refrigerated

Specimen Stability

Room temperature: 7 days
Refrigerated: 5 days
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Hemolysis • Lithium heparin (green-top) tube • Clotted • Received frozen

Methodology
Flow Cytometry (FC)

Setup Schedule

Set up: Mon-Sat; Report available: 2-4 days

Reference Range

Negative

Clinical Significance

HLA-B27 is found in 90% of patients with ankylosing spondylitis and 80% in Reiter's disease. Ankylosing spondylitis affects 1 in 1000 caucasians. Ankylosing spondylitis is 10 times more common among individuals with HLA-B27 compared to individuals without this antigen.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.