Platelet Antibody Screen, Serum

Test Code

LAB767

Quest Code

11484

CPT Codes
86022 (x5)

Preferred Specimen

1.5 mL serum collected in a red-top tube (no gel)

Minimum Volume

0.5 mL

Instructions

Serum should be separated from red cells prior to shipping.

Note: This assay is intended for use as a screening assay. The results of this assay should not be used as the sole basis for a clinical decision. The reaction patterns a test sample produces with this product should not be relied on solely to establish the identity of a platelet antibody. Therefore, positive or negative results obtained using this assay should be used in conjunction with clinical findings or other serological tests.

Some low-titer, low-avidity antibodies may not be detected using this assay.

The presence of other human platelet antigen (HPA) polymorphic variants located on glycoprotein (GP)IIb/IIIa (HPA-6, 7, 8, 9, 10, 11, 14, 16, 17, 19, 20, 21), GPIa/IIa (HPA-13, 18), and GPIb/IX (HPA-12) has not been determined for the antigens captured in this kit. Antibodies to these systems may be reactive in this assay.

Antibodies to low incidence class I human leukocyte antigens may not be detected using this product.

This test has not been evaluated for the detection of autoantibodies to platelet antigens.

Transport Temperature

Frozen

Specimen Stability

Room temperature: Unacceptable
Refrigerated: 48 hours
Frozen: 1 year

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis • Serum separator tube (SST)

Methodology
Solid Phase Enzyme-Linked Immunosorbent Assay (ELISA)

Setup Schedule

Set up: Mon-Fri; Report available: 2-4 days

Reference Range

See Laboratory Report

Clinical Significance

Platelet antibodies may be allo- or autoantibodies and may be directed to a wide range of antigenic "targets" carried on platelet cytoplasmic membranes. Serum platelet antibody test is optimized to identify the presence of platelet alloantibodies in the patient.

Platelet alloantibodies are involved in several clinical situations such as:
- Immune mediated refractoriness to platelet transfusions usually due to antibodies to class I human leukocyte antigens and sometimes to antibodies specific to platelet antigens.
- Neonatal alloimmune thrombocytopenia
- Posttransfusion purpura, which are usually associated with platelet-specific antibodies

This test is not recommended for the diagnosis of immune thrombocytopenia or autoimmune thrombocytopenia. Tests that are optimized to detect antibodies bound to the platelets will be useful in these situations; cell-bound platelet antibody (direct) test is strongly recommended.

Performing Laboratory
Mayo Clinic Laboratories
200 First Street SW
Rochester, MN 55905

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.