| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Cryoglobulin, Qualitative Analysis
Test Code383
CPT Codes
82595
Preferred Specimen
3 mL fasting serum collected in a red-top tube (no gel)
Patient Preparation
Overnight fasting is required
Minimum Volume
2 mL
Instructions
Collect 6 mL of fasting whole blood specimen in a red-top tube (no gel). 3 mL serum required: 2 mL minimum. Avoid hemolysis.
Allow serum sample to clot for one (1) hour in a 37° C water bath, oven, incubator, or heat block. After clotting, centrifuge the specimen for 10 minutes at 1600 RCF(G) utilizing one of the 3 options below:
1. In a "warm" centrifuge at 37° C
2. In a bench-top centrifuge placed in a "warm room" at 37° C
3. At room temperature, in a bench-top centrifuge prewarmed by running it for 10 minutes at 1600 RCF(G).
After centrifuging, decant serum into a screw-capped vial. Transport at room temperature.
Note: If the sample is NOT kept at 37° C during all processing steps, the cryoprecipitate if present can precipitate out of solution and be lost in the clot or during the centrifugation process and result in a false negative result. Transport screw-capped vial at room temperature. DO NOT REFRIGERATE OR FREEZE.
No other tests can be added to the tube; Cryocrit and Cryoglobulin evaluation only.
Allow serum sample to clot for one (1) hour in a 37° C water bath, oven, incubator, or heat block. After clotting, centrifuge the specimen for 10 minutes at 1600 RCF(G) utilizing one of the 3 options below:
1. In a "warm" centrifuge at 37° C
2. In a bench-top centrifuge placed in a "warm room" at 37° C
3. At room temperature, in a bench-top centrifuge prewarmed by running it for 10 minutes at 1600 RCF(G).
After centrifuging, decant serum into a screw-capped vial. Transport at room temperature.
Note: If the sample is NOT kept at 37° C during all processing steps, the cryoprecipitate if present can precipitate out of solution and be lost in the clot or during the centrifugation process and result in a false negative result. Transport screw-capped vial at room temperature. DO NOT REFRIGERATE OR FREEZE.
No other tests can be added to the tube; Cryocrit and Cryoglobulin evaluation only.
Transport Container
Transport tube
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 72 hours
Refrigerated: Unstable
Frozen: Unstable
Refrigerated: Unstable
Frozen: Unstable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Grossly lipemic • Received refrigerated • Received frozen • Icteric samples • Serum separator tube (SST)
Methodology
Visual Inspection
Setup Schedule
Daily
Report Available
4 days
Reference Range
None detected
Clinical Significance
Cryoglobulins are a group of proteins which precipitate from serum, plasma or urine upon exposure to cold.
Performing Laboratory
| Quest Diagnostics Nichols Institute-Chantilly VA |
| 14225 Newbrook Drive |
| Chantilly, VA 20151-2228 |
Last Updated: November 6, 2024
