Chlamydia/Neisseria gonorrhoeae RNA, TMA with Reflex to Alternate Target, Urogenital

Test Code

91773

CPT Codes
87491, 87591

Includes

⁠⁠⁠⁠⁠⁠⁠Chlamydia trachomatis RNA, TMA, Urogenital
Neisseria gonorrhoeae RNA, TMA, Urogenital

If Chlamydia trachomatis RNA, TMA, Urogenital is Detected, then Chlamydia trachomatis, TMA (Alternate Target), Urogenital will be performed at an additional charge (CPT code(s): 87491).
If Neisseria gonorrhoeae RNA, TMA, Urogenital is Detected, then Neisseria gonorrhoeae, TMA (Alternate Target), Urogenital will be performed at an additional charge (CPT code(s): 87591).

Preferred Specimen

1 vaginal swab in Aptima® Transport Tube or
1 endocervical or urethral swab in Aptima® Transport Tube or
2 mL urine in Aptima® Transport Tube or
1 mL liquid cytology ThinPrep® PreservCyt® preservative in Aptima® Transfer Tube or
0.5 mL SurePath™ preservative fluid in Aptima® Transfer Tube

Patient Preparation
Urine: Patient should not have urinated within one hour prior to collection. Female patients should not cleanse the labial area prior to providing the specimen.

Minimum Volume

1 vaginal swab in Aptima® Transport • 1 endocervical or urethral swab in Aptima® Transport • 2 mL urine in Aptima® Transport • 1 mL ThinPrep® PreservCyt® in Aptima® Transfer Tube • 0.5 mL SurePath™ fluid in Aptima® Transfer Tube

Instructions

Vaginal swab: Follow instructions in the Aptima® Multitest Collection Kit (orange label).

Endocervical or urethral swab: Follow instructions in the Aptima® Unisex Swab Specimen Collection Kit (white label). In females, to ensure collection of cells infected with N. gonorrhoeae/C. trachomatis, columnar epithelial cells lining the endocervix should be obtained. Excess mucus should be removed prior to sampling.

Urine: Direct patient to provide a first-catch urine (a maximum of 20-30 mL of the initial urine stream) into a urine collection cup free of any preservatives. 2 mL of urine specimen must be transferred into the Aptima® Specimen Transport within 24 hours of collection and before being assayed. Use the tube provided in the urine specimen collection kit. The fluid (urine plus transport media) level in the urine transport tube must fall within the clear pane of the tube label.

Labs performing cytology: Aliquot ThinPrep® PreservCyt® solution before performance of liquid-based cytology testing.

ThinPrep® PreservCyt®: Transfer 1 mL ThinPrep® PreservCyt® solution into Aptima® Specimen Transfer Tube (green label) or Aptima® Multitest Collection Tube (orange label) or Unisex Swab Specimen Collection Kit (white label).

SurePath™: SurePath™ fluid must be transferred to Aptima® tube within 4 days of collection. Transfer 0.5 mL SurePath™ solution into Aptima® Specimen Transfer Tube (green label) or Aptima® Multitest Collection Tube (orange label) or Unisex Swab Specimen Collection Kit (white label).

For a complete list of assay limitations we recommend that you review the manufacturers package insert, https://www.hologic.com/package-inserts/diagnostic-products/aptima-combo-2-assay-ctng

Transport Container

Aptima® Collection Kit

Transport Temperature

Room temperature

Specimen Stability

Swabs in Aptima® Transport
Room temperature: 60 days
Refrigerated: 60 days
Frozen: 12 months

Urine in Aptima® Transport Medium
Room temperature: 30 days
Refrigerated: 30 days
Frozen: 12 months

Liquid cytology ThinPrep® Preservcyt®:
Room temperature: 14 days
Refrigerated: 30 days
Frozen: 12 months

SurePath™ Preservative Fluid
Room temperature: 14 days
Refrigerated: 14 days
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Transport tubes with 2 swabs • Transport tubes with Non-Aptima® swabs • Swab transport tubes with no swab • Specimens in broken containers • Swab submitted in viral transport media • Urine samples where the fluid level is not between the black fill lines • ThinPrep® PreservCyt® material previously processed for cytology • ThinPrep® PreservCyt® with excess mucus

Methodology
Transcription-Mediated Amplification (TMA)

Setup Schedule

Mon-Sat

Reference Range

C. trachomatis RNA, TMA, Urogenital	Not detected
N. gonorrhoeae RNA, TMA, Urogenital	Not detected

Clinical Significance

To screen for the infection with Neisseria gonorrhoeae and Chlamydia trachomatis in vaginal, endocervical and urethral swabs or urine from men and women.

Performing Laboratory

Quest Diagnostics Nichols Institute-Chantilly VA

14225 Newbrook Drive

Chantilly, VA 20151-2228

Last Updated: November 6, 2024

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.