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CD45 (Leukocyte Common Antigen), IHC with Interpretation
Test Code15798
CPT Codes
88342
Preferred Specimen
Formalin-fixed, paraffin embedded tissue
Other Acceptable Specimens
4-micron unstained slides • Fixed tissue • Tissue in neutral buffered formalin
Instructions
Pathology report is required
Transport Container
IHC specimen transport kit
Transport Temperature
Room temperature
Specimen Stability
Room temperature: Indefinite
Refrigerated: Indefinite
Frozen: Unacceptable
Refrigerated: Indefinite
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received frozen
Methodology
Immunohistochemical Assay (IHC)
Setup Schedule
Mon-sat
Report Available
2-3 days
Clinical Significance
This immunoassay is a first-line screening test to evaluate neoplasms of unknown origin and to help determine whether a cell is derived from a hematopoietic origin. In benign tissue or cell blocks, CD45 can be used as a marker of inflammatory cells. CD45 may be used in the evaluation of lymphomas [1].
The CD45 immunohistochemical stain uses an antibody against a transmembrane glycoprotein expressed on most nucleated hematopoietic cell types. It is used to evaluate cells of hematopoietic lineage. Positive staining is generally indicated by membranous cellular immunoreactivity, but sometimes cytoplasmic immunoreactivity is also seen [1]. The CD45 test will identify most nucleated cells of hematopoietic lineage, including granulocytes, basophils, lymphocytes, mast cells, macrophages, monocytes, and plasma cells. It will not stain platelets, erythrocytes, megakaryocytes, or erythrocyte precursors. Other cell types, including dendritic cells and thymocytes, may also show immunoreactivity to CD45 [1]. In the evaluation of analysis of hematopoietic tumors, the CD45 immunostain will stain neoplastic hematopoietic cells as well as infiltrating non-neoplastic inflammatory cells [1].
This test should be interpreted in the context of pertinent clinical and family history and physical examination findings.
Reference
1. Rekhtman N, et al. Quick Reference Handbook for Surgical Pathologists. 2nd ed. New York, NY: Springer;2011:34.
The CD45 immunohistochemical stain uses an antibody against a transmembrane glycoprotein expressed on most nucleated hematopoietic cell types. It is used to evaluate cells of hematopoietic lineage. Positive staining is generally indicated by membranous cellular immunoreactivity, but sometimes cytoplasmic immunoreactivity is also seen [1]. The CD45 test will identify most nucleated cells of hematopoietic lineage, including granulocytes, basophils, lymphocytes, mast cells, macrophages, monocytes, and plasma cells. It will not stain platelets, erythrocytes, megakaryocytes, or erythrocyte precursors. Other cell types, including dendritic cells and thymocytes, may also show immunoreactivity to CD45 [1]. In the evaluation of analysis of hematopoietic tumors, the CD45 immunostain will stain neoplastic hematopoietic cells as well as infiltrating non-neoplastic inflammatory cells [1].
This test should be interpreted in the context of pertinent clinical and family history and physical examination findings.
Reference
1. Rekhtman N, et al. Quick Reference Handbook for Surgical Pathologists. 2nd ed. New York, NY: Springer;2011:34.
Performing Laboratory
| Quest Diagnostics Nichols Institute-San Juan Capistrano, CA |
| 33608 Ortega Highway |
| San Juan Capistrano, CA 92675-2042 |
Last Updated: November 6, 2024
