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Hepatitis C Virus RNA, Quantitative Real-Time PCR with Reflex to Genotype
Test Code11348
CPT Codes
87522
Includes
If Hepatitis C Viral RNA, Quantitative, Real-Time PCR is ≥2000 IU/mL, then Hepatitis C Virus RNA Genotype will be performed at an additional charge (CPT code(s): 87902).
Preferred Specimen
3 mL plasma collected in an EDTA (lavender-top) tube
Minimum Volume
1.5 mL
Other Acceptable Specimens
Plasma collected in: PPT potassium EDTA (white-top) tube • Serum
Instructions
Separate plasma from whole blood within 24 hours of collection by centrifugation. Transfer to a transport tube and refrigerate or freeze.
Serum is collected in a serum separator tube or red-top tube (no gel). Transfer to a transport tube and refrigerate or freeze.
Transport Container
Transport tube
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 72 hours
Refrigerated: 14 days
Frozen: 30 days
Refrigerated: 14 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Unspun PPT tube • Unspun serum separator tube (SST) • Unspun red-top tube (no gel) • Received room temperature • Gross hemolysis • Grossly lipemic • Specimens collected using heparin as anticoagulant
Methodology
Real-Time Polymerase Chain Reaction
Setup Schedule
Set up: Mon-Sat; Report available: 2-4 days
Reference Range
| HCV RNA, QN, Real-Time PCR | Not detected (IU/mL) |
| HCV RNA, QN, Real-Time PCR | Not detected (Log IU/mL) |
Clinical Significance
Useful in monitoring response to therapy and/or disease progression. Reportable range is 15 to 100,000,000 IU/mL (1.18-8.00 log IU/mL).
Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153
Last Updated: May 19, 2016
