Cytomegalovirus (CMV) Genotype (UL97/UL54)

Test Code

13948

CPT Codes
87910, 87900

Includes

Ganciclovir Resistance
Maribavir Resistance
Foscarnet Resistance
Cidofovir Resistance
UL97 Kinase Mutations
UL54 Polymerase Mutations

Preferred Specimen

1 mL whole blood collected in an EDTA (lavender-top) tube or
1 mL plasma collected in an EDTA (lavender-top) tube or PPT tube

Minimum Volume

0.3 mL

Instructions

Whole blood: Collect whole blood in sterile tubes containing EDTA as anticoagulant. Store refrigerated or frozen.
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Plasma: Collect blood in sterile tubes containing EDTA as anticoagulant or in PPT tubes. Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile plastic screw-cap tubes and store refrigerated or frozen. If blood is collected in a plasma preparation tube, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a plasma preparation tube to aliquot tubes.

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: 48 hours
Refrigerated: 8 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Samples containing heparin • Unspun PPT tubes

Methodology
Polymerase Chain Reaction (PCR) • Next Generation Sequencing (NGS)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Mon, Wed; Report available: 5-8 days

Reference Range

See Laboratory Report

Clinical Significance

Treatment of CMV diseases includes five licensed drugs: Ganciclovir, Maribavir, Foscarnet, Cidofovir, and Letermovir. These drugs inhibit the viral phosphotransferase, DNA polymerase or terminase through various mechanisms. Over time, as CMV makes copies of itself, viral genes can acquire mutations. These mutations may make CMV resistant to the effects of antiviral drugs. Therefore, it is important to detect resistance as quickly and accurately as possible for proper management of CMV infection.

Performing Laboratory
Quest Diagnostics Nichols Institute (IFD) SJC
33608 Ortega Highway
San Juan Capistrano, CA 92675

Last Updated: March 5, 2025

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.