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CD21, IHC without Interpretation
Test Code40016X
Preferred Specimen
Formalin fixed paraffin embedded tissue in an IHC specimen transport kit
Other Acceptable Specimens
4-micron unstained slides (x5) • Fixed tissue
Instructions
Tumor paraffin block (formalin-fixed only) or five 4-micron unstained sections on Poly-L-Lysine or Silane
coated slides. See Specimen Collection Section, Oncology. Ship at room temperature. Do not place
paper labels with adhesive backing on slides. Use pencil or xylene resistant pen to write on the
frosted end of the slide only. Pathology report is required.
coated slides. See Specimen Collection Section, Oncology. Ship at room temperature. Do not place
paper labels with adhesive backing on slides. Use pencil or xylene resistant pen to write on the
frosted end of the slide only. Pathology report is required.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: Indefinite
Refrigerated: Indefinite
Frozen: Unacceptable
Refrigerated: Indefinite
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Tissue in formalin
Methodology
Immunohistochemistry (IHC)
Setup Schedule
A.M.
Clinical Significance
CD21 antigen is a type I integral membrane glycoprotein of molecular weight 140 kD, which functions as
the receptor for the C3d fragment of the third complement component. The CD21 molecule, present on
mature B cells, is involved in transmitting growth-promoting signals to the interior of the B cell and acts
as a receptor for Epstein-Barr virus. CD21 antigen is reported to be found in B cell chronic lymphocytic
leukemias and in a subset of T cell acute lymphocytic leukemias, but is absent on T lymphocytes, monocytes and granulocytes. CD21 antigen is also reported to be expressed in follicular dendritic cells
and in follicular and centrocytic lymphomas, mature leukemias and lymphomas and at high density in
follicular dendretic cells.
the receptor for the C3d fragment of the third complement component. The CD21 molecule, present on
mature B cells, is involved in transmitting growth-promoting signals to the interior of the B cell and acts
as a receptor for Epstein-Barr virus. CD21 antigen is reported to be found in B cell chronic lymphocytic
leukemias and in a subset of T cell acute lymphocytic leukemias, but is absent on T lymphocytes, monocytes and granulocytes. CD21 antigen is also reported to be expressed in follicular dendritic cells
and in follicular and centrocytic lymphomas, mature leukemias and lymphomas and at high density in
follicular dendretic cells.
