Steroid Panel, Comprehensive

Test Code

90392

Includes

11-Deoxycortisol
17-Hydroxyprogesterone
17-Hydroxypregnenolone
18-Hydroxycorticosterone and Cortisone
Androstenedione
Corticosterone
Cortisol
DHEA, Unconjugated
Deoxycorticosterone
Pregnenolone
Progesterone
Testosterone, Total, LC/MS/MS

Preferred Specimen

0.5 mL frozen serum collected in a red-top tube (no gel)

Minimum Volume

0.25 mL

Instructions

Collect blood in a Vacutainer® with no additives. Allow blood to clot (30 minutes) at room temperature, 18-25°C. Centrifuge and separate the serum from the cells. Freeze as soon as possible.

Transport Temperature

Frozen

Specimen Stability

Room temperature: Unacceptable
Refrigerated: 72 hours
Frozen -20° C: 28 days
Frozen -70° C: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis • Moderate hemolysis • Grossly lipemic • Grossly icteric • Serum separator tube (SST) • Plasma

Methodology
Chromatography/Mass Spectrometry

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Night

Clinical Significance

This panel identifies all major adrenal steroid hormones and facilitates the diagnosis of the four enzymatic defects associated with congenital adrenal hyperplasia (CAH). All steroids are determined in a single assay by LCMSMS using as little as 0.1 mL serum.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.