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Direct Coombs (Anti-Human Globulin)
MessageTest Code
4550
Alias/See Also
DAT [Direct Coombs (Anti-Human Globulin)]; Direct Antihuman Globulin Test [Direct Coombs (Anti-Human Globulin)]; Direct Antiglobulin Test [Direct Coombs (Anti-Human Globulin)]; Coombs, Direct [Direct Coombs (Anti-Human Globulin)]
Preferred Specimen
One 10 mL lavender (EDTA) .
Minimum Volume
10 mL whole blood at 2-8°C.
Instructions
Transport Temperature
10 mL whole blood at 2-8°C.
Specimen Stability
Ambient: 3 days; Refrigerated: 1 week; Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Separator or gel tubes.
Methodology
Hemagglutination
Setup Schedule
Mon-Fri
Report Available
1-3 days
Limitations
Do not freeze. For Pediatric Collect: 1 mL whole blood at 2-8°C.
Clinical Significance
This direct Coombs test should be performed with polyspecific Coombs sera. If the test is positive, then additional testing should be performed using monospecific Coombs seras.
