Antimicrobial Susceptibility - MIC/MBC

Message

The MIC is defined as the lowest concentration of an antibiotic which will inhibit the (in vitro) growth of an infectious organism. Results are reported in micrograms per mL. The interpretation of in vitro data is based on achievable serum concentrations, which may vary depending on dose, route of administration, degree of protein binding, site of infection, age and weight of the patient, and other factors.The MBC is defined as the lowest concentration of an antimicrobial agent needed to kill 99.9% of the initial organism inoculum. Presently, specific guidelines for interpretation are not available; therefore, a clinician knowledgeable in both bactericidal testing and infectious diseases should be available to interpret the results.For serious infections with coagulase-negative staphylococci, testing for the presence of mecA will be performed in order to interpret the results for agents.This test must be ordered for each antimicrobial agent tested.The minimum turnaround time is 48 hours for nonfastidious, rapidly growing organisms and greater than or equal to 96 hours for fastidious, slow growing organisms.An additional handling fee will be billed for all organisms submitted that are not in pure culture as indicated in the specimen requirements.

Test Code

6200

Alias/See Also

MIC/ID; MIC; MIC/MBC (Antimicrobial Susceptibility - MIC/MBC); Minimum Inhibitory Concentration (MIC)/Minimum Bactericidal Concentration (MBC) (Antimicrobial Susce; Antibiotic MIC/MBC (Antimicrobial Susceptibility - MIC/MBC)

Preferred Specimen

Actively growing isolated organism, in pure culture, on agar slant or in bacterial transport media. Indicate isolate identification and specimen source.

Minimum Volume

Organism in pure culture in a sealed container at 20-25°C. Submit specimen according to Biological Substance, Category B, shipping guidelines.

Transport Temperature

Organism in pure culture in a sealed container at 20-25°C. Submit specimen according to Biological Substance, Category B, shipping guidelines.

Specimen Stability

Ambient: 1 week; Refrigerated: Unacceptable; Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Mixed cultures or nonviable organisms.

Methodology
Broth Microdilution

Setup Schedule

Sun-Sat

Report Available

2-4 days

Limitations

Refer to Note below. Individual antibiotics must be specified.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.