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Fungal Culture
MessageIf Histoplasma, Coccidioides, or Blastomyces is the presumptive diagnosis, a DNA probe will be added for confirmation at an additional charge per probe.Identification performed on mould isolates. Limited yeast identification performed from non-sterile sites. Identification of moulds and/or yeasts on positives is billed separately from culture.Fungal smear must be ordered separately. Refer to Fungal Stain, KOH with Calcofluor White (ARUP test code 2004589).Order test according to source type:Blood/Bone Marrow: Blood Culture, Fungal (ARUP test code 0060070)Genital, fecal and oral: Yeast Culture (ARUP test code 0060150)Skin, hair and nails: Fungal Culture, Skin, Hair and Nails (ARUP test code 0060728)All other sources: Fungal Culture (ARUP test code 0060149)
Test Code
6090
Alias/See Also
Culture, Fungus (Fungal Culture); Yeast Culture; Mold Culture (Fungal Culture); Culture, Yeast; Skin Culture (Fungal Culture)
Preferred Specimen
Material or fluid from any body site.
Minimum Volume
Specimens from a sterile body site (fluids, tissues, etc.): Room temperature. Specimens from a nonsterile site (respiratory, GI tract, etc.): Refrigerated. If CSF cannot be transported within eight hours, hold CSF at 35°C until the time of transport.
Instructions
Place each specimen in a separate, individually sealed bag. (Min: 1 mL fluid) A single specimen may be cultured for both bacteria and fungi. Source of specimen is required. Also acceptable: Swabs (but sub-optimal for recovery of fungi).
Transport Temperature
Specimens from a sterile body site (fluids, tissues, etc.): Room temperature. Specimens from a nonsterile site (respiratory, GI tract, etc.): Refrigerated. If CSF cannot be transported within eight hours, hold CSF at 35°C until the time of transport.
Specimen Stability
Ambient: 24 hours; Refrigerated: 1 week; Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Catheter tips.
Methodology
Standard reference procedures for fungal culture. Standard reference procedures for identification and/or ITS rDNA Sequencing. DNA probes available for Histoplasma, Coccidioides, and Blastomyces
Setup Schedule
Sun-Sat
Report Available
Positive cultures are reported as soon as detected Preliminary negative within 3 weeksFinal negative within 5 weeks
Limitations
Please notify laboratory if Malassezia furfur is suspected. Special media must be used for the cultivation of this yeast.