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AFB Culture (Includes AFB Stain 0060151)
MessageAFB stain, AFB identification of positives, and drug susceptibility tests are billed separately from culture. Identification of positive culture is billed by individual DNA probes and tests performed. Mycobacterium tuberculosis Amplified Direct Detection (0060095) is available for respiratory specimens. For CSF specimens, order Mycobacterium tuberculosis Amplified Detection, CSF (0060063). The laboratory should be notified when the presence of Mycobacterium genavense is suspected as this organism will not grow on media routinely used for Mycobacterium isolation.For AFB culture on blood refer to Blood Culture, AFB (0060060).
Test Code
6010
Alias/See Also
Mycobacteria Culture [AFB Culture (Includes AFB Stain 0060151)]; MTB Culture [AFB Culture (Includes AFB Stain 0060151)]; TB Culture [AFB Culture (Includes AFB Stain 0060151)]; Blood Culture, AFB [AFB Culture (Includes AFB Stain 0060151)]; Culture, AFB [AFB Culture (Includes AFB Stain 0060151)]
Preferred Specimen
Sputum, CSF, urine, body fluid, tissue, OR gastric aspirate.
Minimum Volume
Refrigerated. Submit specimen according to Biological Substance, Category B, shipping guidelines.
Instructions
Three sputum specimens should be collected at 8-24 hour intervals (24 hours when possible) and should include at least one first-morning specimen. Gastric lavage specimens must be neutralized with sodium carbonate if transport is delayed for more than four hours. Source of specimen is required. Transfer (for each collection) 10-15 mL sputum (Min: 1 mL), 5 mL CSF (Min: 1 mL), at least 40 mL urine (Min: 10 mL), at least 5 mL body fluid (Min: 1 mL), tissue (Min: Visible), OR 5-10 mL gastric aspirate (Min: 1 mL) to a sterile, leak-proof container. Place each specimen in an individual sealed bag.
Transport Temperature
Refrigerated. Submit specimen according to Biological Substance, Category B, shipping guidelines.
Specimen Stability
Ambient: 24 hours; Refrigerated: 1 week; Frozen: 1 week
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Multiple same-site specimens (more than one in 24 hours), dry material, or material collected and transported on a SWAB.
Methodology
Standard reference procedures for stain and culture. Identification tests of AFB are ordered and billed separately. DNA probes are available for M. tuberculosis complex and M. avium-intracellulare complex as indicated. DNA sequencing and other molecular techniques are used for identification. For drug susceptibilities, refer to Antimicrobial Susceptibility - AFB Mycobacteria (ARUP test code 0060217).
Setup Schedule
Sun-Sat
Report Available
1-62 daysStain: 24 hours; Final negative after 8 weeksPositive cultures are reported as soon as detected.
Limitations
Acid fast stain will NOT be performed on stool specimens, CSF if less than 5 mL, or urine specimens if less than 40 mL. However, these specimens will be cultured.
