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Kleihauer-Betke Stain for Fetal Hemoglobin
MessageThis test should be ordered on pregnant or post-partum women who need to be assessed for RhoGAM® or fetal-maternal bleeds. For routine fetal hemoglobin (Hb F) testing, please order Hemoglobin Evaluation with Reflex to Electrophoresis and/or HPLC (0050610).
Test Code
860
Alias/See Also
Maternal fetal bleed; Fetal Hemoglobin, Semi-Quantitative
Preferred Specimen
One 3 mL lavender (EDTA) or pink (K2EDTA).
Minimum Volume
3 mL whole blood at 2-8°C. (Min: 0.5 mL)
Transport Temperature
3 mL whole blood at 2-8°C. (Min: 0.5 mL)
Specimen Stability
Ambient: 3 days; Refrigerated: 3 days; Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Frozen specimens, clotted specimens, and specimens collected after Rh immunoglobulin administered.
Methodology
Semi-Quantitative Acid Elution Eosin Stain/Microscopy
Setup Schedule
Sun-Sat
Report Available
Within 24 hours
Clinical Significance
Volume (mL) of fetomaternal hemorrhage (assuming a maternal blood volume of 5 liters) = percent fetal rbcs x50. The quantitation of fetomaternal hemorrhage by this method is inherently imprecise. Therefore, it is important to provide a safety margin when calculating the dose of RhIG.
