CULTURE BLOOD

CXBLD

LAB462 BLOOD CULTURE 84066

By convention, should always include 2 sets. An order is needed for each set. A set is comprised of one aerobic bottle (blue or green) and one anaerobic bottle (purple or orange), both drawn peripherally from two different collection sites.

Peripheral draw, 10 mL per bottle for adults; Peds: 0.5 - 4 mL single PF bottle (yellow label).

Adults: 7 ml per bottle. Peds: 0.5

Prep skin venipuncture site per laboratory-approved SOP. Collect 20 ml blood and inject 10 ml each into one anerobic and one aerobic bottle. Repeat procedure from a new venipuncture site. Apply labels lengthwise on bottles. Initial, date/time on each bottle. Indicate blood collection site on label. Note: For adults, each set should contain one of the following combinations, depending on bottle type availability: Green/Purple; Green/Orange; Blue/Purple; Blue/Orange. For short draws, inject 10 ml in the aerobic bottle (green or blue) and any remaining volume into the anaerobic bottle (purple or orange).

Bio Merieux BacT/ALERT bottle types only. A set consists of one aerobic (either blue or green) - AND - one anaerobic (either purple or orange). For pediatrics, only a single yellow (PF) bottle is required.

Label with patient's full name and date of birth or LIS label and submit to Microbiology immediately. Transport at ambient temperature. Do not refrigerate.

Delays in transport are not acceptable as bacterial survival rates outside the body vary based on species. Culture bottles must be loaded in the instrument for continuous monitoring ASAP.

Blood cultures processed continuously, 24/7.

No growth cultures updated every 24 hours around the clock. Final no growth cultures reported after 5 days incubation. Positive cultures monitored and reported 24/7.

Does not include culture for AFB, fungus or viruses.