ANA by IFA, Synovial Fluid (RDL)

Test Code

93964

CPT Codes
86038

Includes

If ANA is positive, then ANA Titer and Pattern will be performed at an additional charge (CPT code(s): 86039).

Preferred Specimen

1 mL synovial fluid collected in a sterile container

Minimum Volume

0.3 mL

Instructions

Note: This volume does not allow for repeat testing

Transport Temperature

Refrigerate (cold packs)

Specimen Stability

Room temperature: 7 days
Refrigerated: 14 days
Frozen: 60 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis • Bacterial contamination • Lipemia • Icteric • Non-synovial body fluids

Methodology
Immunofluorescence Assay

Setup Schedule

Set up: Daily; Report available: 7-14 days

Reference Range

Negative

Homogeneous Pattern: < 1:40
Nucleolar Pattern: < 1:40
Speckled Pattern: < 1:40

Clinical Significance

ANA are commonly found in a variety of autoimmune diseases. Antibody frequency increases with age in apparently healthy people. ANA patterns on Hep-2 slides provide only general clues about particles (chromatin, nucleosomes, and spliceosomes). ANA patterns (other than centromere pattern) are not reliably correlated with the presence of specific antibodies and must be further evaluated by EIA using individual ENA antigens.

Performing Laboratory
Esoterix Endocrinology
4301 Lost Hills Road
Calabasas Hills, CA 91301

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.