Hepatitis C Antibody and HIV-1/2, Screen and Diagnostic Panel with Reflexes

Test Code

36646

CPT Codes
86803, 87389

Includes

If Hepatitis C Antibody is reactive or borderline, then Hepatitis C Viral RNA, Quantitative, Real-Time PCR will be performed at an additional charge (CPT code(s): 87522).
If Hepatitis C Viral RNA, Quantitative, Real-Time PCR is ≥300 IU/mL, then Hepatitis C Viral RNA Genotype, LiPA will be performed at an additional charge (CPT code(s): 87902).

If HIV Antigen and Antibody, 4th Generation Screen is Repeatedly Reactive, then HIV-1/2 Antibody Differentiation will be performed at an additional charge (CPT code(s): 86701, 86702).
If HIV-1/2 Antibody Differentiation is Indeterminate or Negative, then HIV-1 RNA, Qualitative, TMA will be performed at an additional charge (CPT code(s): 87535).

Preferred Specimen

HCV: 2.5 mL serum collected in each of (x2) separate serum separator tubes (SST), and
HIV: 3 mL serum collected in a third separate serum separator tubes (SST)

Minimum Volume

2 mL per SST

Instructions

HIV Serum: Collect in a serum separator tube. The tube should be centrifuged after clotting. Spun serum separator tubes may be submitted at room temperature or refrigerated unopened without transfer to a plastic screw-cap vial.

Tubes submitted for HIV testing must be labeled with patient identifier and submitted only for HIV testing.

Transport Container

See Instructions

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: 72 hours
Refrigerated: 5 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis • Frozen serum separator tube (SST)

Methodology
Immunoassay (IA)

Setup Schedule

Set up and Report Available: See individual assays

Report Available

5 Days

Reference Range

See Laboratory Report

Clinical Significance

HCV: Hepatitis C Virus (HCV) is a major cause of hepatitis. Making positive HCV Ab tests reflex to confirmatory molecular testing is necessary to confirm active HCV infection. Providing a second reflex to perform genotyping on HCV isolates when RNA is detected is also a necessary step before starting therapy. This double reflex will reduce the percentage of antibody-positive patients who lack appropriate confirmatory RNA testing and who do not have genotype testing performed.
HIV: This 4th generation HIV Ag/Ab Combo assay is intended to be used as an aid in the diagnosis of HIV1/HIV2 infection, including acute or primary HIV-1 infection.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.