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RNA, REAL TIME PCR (NON-GRAPH)
Test Code550430
Alias/See Also
"HIV-1 Plasma Viremia
HIV-1 RNA by Real-time PCR, Quantitative
Viral Load
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HIV-1 RNA by Real-time PCR, Quantitative
Viral Load
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CPT Codes
87536
Preferred Specimen
"Plasma
3.5 mL
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3.5 mL
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Minimum Volume
"1.1 mL (Note: This volume does not allow for repeat testing.)
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Instructions
"Collect specimen in lavender-top (EDTA) tube or PPT™. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Remove plasma, transfer plasma to a screw-cap polypropylene transport tube (not “pop top” or “snap cap”), and freeze. Ship frozen (preferred). Do not ship frozen in PPT™. If PPT™ is used to collect plasma, specimen must be transferred to a screw-cap tube prior to freezing to avoid false elevation of low viral load samples. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
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Transport Container
"Ship in a screw-cap polypropylene frozen transport tube.
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Transport Temperature
freeze
Specimen Stability
"Freeze.Stable at room temperature for up to one day or refrigerated for up to five days. Freeze for long-term storage.
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Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
"Hemolysis; green-top (heparin) tube; specimen received in “pop top” or “snap cap” tube; quantity not sufficient for analysis; frozen PPT™
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Methodology
"COBAS® AmpliPrep/COBAS® TaqMan® HIV-1 Test, Version 2.0 "
Limitations
"Assay range: 20-10,000,000 copies/mL
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Clinical Significance
"Detect and quantitate HIV-1 in plasma
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