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Chromosome, Blood, Routine
MessagePertinent medical findings must accompany request for chromosome analysis. Include the patient's name, age, and suspected diagnosis.
Test Code
511035
Alias/See Also
Karyotype; Routine G-Banding; Stimulated Lymphocyte
Preferred Specimen
Blood
Minimum Volume
2 mL
Instructions
Venipuncture: Thoroughly cleanse the area with a Betadine scrub or similar preparation followed by a rinse with 70% alcohol (ethanol may be used instead of isopropyl and is less toxic). Collect 1-10 mL of blood into a heparinized tube. Invert the tube several times to prevent coagulation. If a Vacutainer is used, let off any remaining vacuum by inserting a hypodermic needle or loosening the top briefly. Skin lancet puncture: Thoroughly cleanse the site (finger, earlobe, or newborn child's heel) by the above method. Rinse a sterile, cottonplugged Pasteur pipet or TB syringe with approximately 0.5 mL of sterile heparin, and aspirate blood into it. Care must be taken to use only that blood within the sterilized site; dripping blood may be removed with a sterile gauze pad. The blood-heparin mixture may be placed into a sterile tube for transport. Specimens must be sent the same day as collected and arrive in the laboratory Monday through Saturday and within 72 hours of venipuncture.
Transport Container
Green-stopper (sodium heparin) tube
Transport Temperature
Specimen should be delivered to the laboratory IMMEDIATELY. Maintain specimen AT ROOM TEMPERATURE. Specimens may be refrigerated if there is a delay in shipment. DO NOT FREEZE.
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis; specimen clotted, use of improper anticoagulant; frozen specimen
Methodology
Lymphocyte culture, mitogen stimulation, colchicine arrest of cells in mitosis with predominantly early metaphases, methanol/acetic acid fixation, spread preparation, trypsin- Giemsa banding, chromosome analysis of 20 metaphases with preparation and analy
